Colorimetric detection of active botulinum neurotoxin using Cu2+ mediated gold nanoparticles agglomeration

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Original languageEnglish
Pages (from-to)563-567
Journal / PublicationSensors and Actuators, B: Chemical
Online published24 May 2016
Publication statusPublished - 1 Nov 2016


We report a colorimetric detection of functional botulinum neurotoxin (BoNT) based on its proteolytic activity. Active BoNT can enzymatically cleave SNAptide, a short peptide with 15 amino acids. The cleaved peptides subsequently induced the agglomeration of gold nanoparticles, allowing a colorimetric signal that can be discerned by the naked eye at the low concentration of 1 ng/mL (6.67 pM), and achieve low limit of detection at 0.25 ng/mL (1.67 pM 3 σ/s) by spectral absorbance. This colorimetric detection will provide a simple and promising method for applications at low-resource sites.

Research Area(s)

  • Botulinum neurotoxin, Gold nanoparticles, Colorimetric