Colorimetric and ultra-sensitive fluorescence resonance energy transfer determination of H2O2 and glucose by multi-functional Au nanoclusters

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Qian Zhao
  • Shenna Chen
  • Haowen Huang
  • Lingyang Zhang
  • Linqian Wang
  • Fengping Liu
  • Jian Chen
  • Yunlong Zeng

Detail(s)

Original languageEnglish
Pages (from-to)1498-1503
Journal / PublicationAnalyst
Volume139
Issue number6
Online published5 Dec 2013
Publication statusPublished - 21 Mar 2014

Abstract

Ultra-sensitive colorimetric determination of H2O2 is accomplished based on the intrinsic peroxidase-like activity of Au nanoclusters (AuNCs) stabilized by glutathione (GSH). The color change of 3,3,5,5-tetramethylbenzidine (TMB) catalyzed by AuNCs offers an indirect method to measure glucose. This sensing platform makes use of a dual optical signal change, including the color change in an aqueous solution under visible light illumination and an ultra-sensitive fluorescent assay arising from efficient fluorescence resonance energy transfer (FRET) between the AuNCs and oxidized TMB. The detection limits of H2O2 and glucose are 4.9 × 10-13 M and 1.0 × 10-11 M, respectively. In addition, enhanced fluorescence is observed from the AuNCs due to the use of ethanol which produces clear changes in the quantum yield and lifetime of the AuNCs. The quantum yield of AuNCs is enhanced from ∼12.5% as an isolated fluorophore to 38.9% in an AuNCs-ethanol complex. The enhanced fluorescence lowers the detection limits of H2O2 and glucose by 2 orders of magnitude compared to those attained from the original AuNCs. © 2014 The Royal Society of Chemistry.

Citation Format(s)

Colorimetric and ultra-sensitive fluorescence resonance energy transfer determination of H2O2 and glucose by multi-functional Au nanoclusters. / Zhao, Qian; Chen, Shenna; Huang, Haowen; Zhang, Lingyang; Wang, Linqian; Liu, Fengping; Chen, Jian; Zeng, Yunlong; Chu, Paul K.

In: Analyst, Vol. 139, No. 6, 21.03.2014, p. 1498-1503.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal