Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain

Xuemei Yang; Ning Dong; Xiaoxuan Liu; Chen Yang; Lianwei Ye; Edward Wai-Chi Chan; Rong Zhang; Sheng Chen

doi:10.3389/fmicb.2021.739461

Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain

Xuemei Yang
, Ning Dong
, Xiaoxuan Liu
, Chen Yang
, Lianwei Ye
, Edward Wai-Chi Chan
, Rong Zhang
, Sheng Chen^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

30 Citations (Scopus)

69 Downloads (CityUHK Scholars)

Abstract

Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains have been increasingly reported, and it is important to understand the evolutionary mechanisms of these highly pathogenic and resistant bacterial pathogens. In this study, we characterized a ST11 carbapenem-resistant K. pneumoniae strain which harbored an IncFIB/IncHI1B type virulence plasmid and an IncFII/IncR type bla_KPC_–₂-bearing plasmid. The virulence plasmid was found to be conjugative and harbored a 35-kbp fragment including aerobactin encoding cluster from virulence plasmid pLVPK and multiple resistance genes, resulting in a mosaic multi-drug resistance and virulence plasmid. This virulence plasmid could be transferred via conjugation to Escherichia coli and K. pneumoniae strains alone as well as together with the bla_KPC_–₂-bearing plasmid. Co-transmission of virulence and bla_KPC_–₂-bearing plasmids would directly convert a classic K. pneumoniae strain into CR-HvKP strain, leading to a sharp increase in the prevalence of CR-HvKP in clinical settings, which poses a great threat to human health.

Original language	English
Article number	739461
Journal	Frontiers in Microbiology
Volume	12
Online published	8 Nov 2021
DOIs	https://doi.org/10.3389/fmicb.2021.739461
Publication status	Published - Nov 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

blaKPC-bearing plasmid
conjugative virulence plasmid
CR-HvKP
IncFIB/IncHI1B
IncFII/IncR
Klebsiella pneumonia
transmission

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title = "Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain",

abstract = "Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains have been increasingly reported, and it is important to understand the evolutionary mechanisms of these highly pathogenic and resistant bacterial pathogens. In this study, we characterized a ST11 carbapenem-resistant K. pneumoniae strain which harbored an IncFIB/IncHI1B type virulence plasmid and an IncFII/IncR type blaKPC–2-bearing plasmid. The virulence plasmid was found to be conjugative and harbored a 35-kbp fragment including aerobactin encoding cluster from virulence plasmid pLVPK and multiple resistance genes, resulting in a mosaic multi-drug resistance and virulence plasmid. This virulence plasmid could be transferred via conjugation to Escherichia coli and K. pneumoniae strains alone as well as together with the blaKPC–2-bearing plasmid. Co-transmission of virulence and blaKPC–2-bearing plasmids would directly convert a classic K. pneumoniae strain into CR-HvKP strain, leading to a sharp increase in the prevalence of CR-HvKP in clinical settings, which poses a great threat to human health.",

keywords = "blaKPC-bearing plasmid, conjugative virulence plasmid, CR-HvKP, IncFIB/IncHI1B, IncFII/IncR, Klebsiella pneumonia, transmission",

author = "Xuemei Yang and Ning Dong and Xiaoxuan Liu and Chen Yang and Lianwei Ye and Chan, \{Edward Wai-Chi\} and Rong Zhang and Sheng Chen",

year = "2021",

month = nov,

doi = "10.3389/fmicb.2021.739461",

language = "English",

volume = "12",

journal = "Frontiers in Microbiology",

issn = "1664-302X",

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TY - JOUR

T1 - Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain

AU - Yang, Xuemei

AU - Dong, Ning

AU - Liu, Xiaoxuan

AU - Yang, Chen

AU - Ye, Lianwei

AU - Chan, Edward Wai-Chi

AU - Zhang, Rong

AU - Chen, Sheng

PY - 2021/11

Y1 - 2021/11

N2 - Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains have been increasingly reported, and it is important to understand the evolutionary mechanisms of these highly pathogenic and resistant bacterial pathogens. In this study, we characterized a ST11 carbapenem-resistant K. pneumoniae strain which harbored an IncFIB/IncHI1B type virulence plasmid and an IncFII/IncR type blaKPC–2-bearing plasmid. The virulence plasmid was found to be conjugative and harbored a 35-kbp fragment including aerobactin encoding cluster from virulence plasmid pLVPK and multiple resistance genes, resulting in a mosaic multi-drug resistance and virulence plasmid. This virulence plasmid could be transferred via conjugation to Escherichia coli and K. pneumoniae strains alone as well as together with the blaKPC–2-bearing plasmid. Co-transmission of virulence and blaKPC–2-bearing plasmids would directly convert a classic K. pneumoniae strain into CR-HvKP strain, leading to a sharp increase in the prevalence of CR-HvKP in clinical settings, which poses a great threat to human health.

AB - Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains have been increasingly reported, and it is important to understand the evolutionary mechanisms of these highly pathogenic and resistant bacterial pathogens. In this study, we characterized a ST11 carbapenem-resistant K. pneumoniae strain which harbored an IncFIB/IncHI1B type virulence plasmid and an IncFII/IncR type blaKPC–2-bearing plasmid. The virulence plasmid was found to be conjugative and harbored a 35-kbp fragment including aerobactin encoding cluster from virulence plasmid pLVPK and multiple resistance genes, resulting in a mosaic multi-drug resistance and virulence plasmid. This virulence plasmid could be transferred via conjugation to Escherichia coli and K. pneumoniae strains alone as well as together with the blaKPC–2-bearing plasmid. Co-transmission of virulence and blaKPC–2-bearing plasmids would directly convert a classic K. pneumoniae strain into CR-HvKP strain, leading to a sharp increase in the prevalence of CR-HvKP in clinical settings, which poses a great threat to human health.

KW - blaKPC-bearing plasmid

KW - conjugative virulence plasmid

KW - CR-HvKP

KW - IncFIB/IncHI1B

KW - IncFII/IncR

KW - Klebsiella pneumonia

KW - transmission

UR - https://www.scopus.com/pages/publications/85119613869

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85119613869&origin=recordpage

U2 - 10.3389/fmicb.2021.739461

DO - 10.3389/fmicb.2021.739461

M3 - RGC 21 - Publication in refereed journal

C2 - 34819921

SN - 1664-302X

VL - 12

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 739461

ER -

Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain

Abstract

UN SDGs

Research Keywords

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