Cluster Nanozymes with Optimized Reactivity and Utilization of Active Sites for Effective Peroxidase (and Oxidase) Mimicking

Jieru Zhang, Tai-Sing Wu, Ho Viet Thang, Kai-Yu Tseng, Xiaodong Hao*, Bingshe Xu, Hsin-Yi Tiffany Chen*, Yung-Kang Peng*

*Corresponding author for this work

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

29 Citations (Scopus)

Abstract

Single-atom catalysts have attracted attention in the past decade since they maximize the utilization of active sites and facilitate the understanding of product distribution in some catalytic reactions. Recently, this idea has been extended to single-atom nanozymes (SAzymes) for the mimicking of natural enzymes such as horseradish peroxidase (HRP) often used in bioanalytical applications. Herein, it is demonstrated that those SAzymes without constructing the reaction pocket of HRP still undergo the OH radical-mediated pathway like most of the reported nanozymes. Their positively charged single-atom centers resulting from support electronegative oxygen/nitrogen hinder the reductive conversion of H2O2 to OH radicals and hence display low activity per site. In contrast, it is found that this step can be facilitated over their metallic counterparts on cluster nanozymes with much higher site activity and atom efficiency (cf. SAzymes with 100% atom utilization). Besides the mimicking of HRP in glucose detection, cluster nanozymes are also demonstrated as a better oxidase mimetic for glutathione detection.
Original languageEnglish
Article number2104844
JournalSmall
Volume18
Issue number5
Online published25 Nov 2021
DOIs
Publication statusPublished - 3 Feb 2022

Research Keywords

  • active site reactivity
  • atom utilization
  • cluster nanozymes
  • peroxidase/oxidase mimicking
  • single-atom nanozymes

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