Cloning and expression analysis of the pcbAB-pcbC β-lactam genes in the marine fungus Kallichroma tethys

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

18 Scopus Citations
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Author(s)

  • Chi-Fai Kim
  • Simon K. Y. Lee
  • Jackie Price
  • Ralph W. Jack
  • Geoffrey Turner

Detail(s)

Original languageEnglish
Pages (from-to)1308-1314
Journal / PublicationApplied and Environmental Microbiology
Volume69
Issue number2
Publication statusPublished - 1 Feb 2003

Abstract

Here we report the identification of the β-lactam biosynthesis genes pcbAB and pcbC from a cosmid genomic DNA library of the marine fungus Kallichroma tethys. A BLAST homology search showed that they share high sequence identity with the δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetases and isopenicillin N synthases, respectively, of various fungal and bacterial β-lactam producers, while phylogenetic analysis indicated a close relationship with homologous genes of the cephalosporin-producing pyrenomycete Acremonium chrysogenum. Expression analysis by reverse transciption-PCR suggested that both genes are highly regulated and are expressed in the late growth phase of K. tethys cultures. Complementation of an Aspergillus nidulans strain deficient in ACV synthetase suggested that at least pcbAB is functional, although attempts to isolate active antibiotic from K. tethys were unsuccessful.

Citation Format(s)

Cloning and expression analysis of the pcbAB-pcbC β-lactam genes in the marine fungus Kallichroma tethys. / Kim, Chi-Fai; Lee, Simon K. Y.; Price, Jackie; Jack, Ralph W.; Turner, Geoffrey; Kong, Richard.Y.C.

In: Applied and Environmental Microbiology, Vol. 69, No. 2, 01.02.2003, p. 1308-1314.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal