CIRCULATING TUMOR CELL (CTC) ENRICHMENT : ULTRA HIGH THROUGHPUT PROCESSING OF CLINICALLY RELEVANT BLOOD VOLUMES USING A MULTIPLEXED SPIRAL BIOCHIP

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

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Author(s)

  • M.E. Warkiani
  • D.S.W. Tan
  • A.A.S. Bhagat
  • W.T. Lim
  • J. Han
  • C.T. Lim

Detail(s)

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
PublisherChemical and Biological Microsystems Society
Pages1156-1158
Volume1
ISBN (Print)9781632666246
Publication statusPublished - Oct 2013
Externally publishedYes

Conference

Title17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
LocationMesse Freiburg
PlaceGermany
CityFreiburg
Period27 - 31 October 2013

Abstract

Detection and characterization of circulating tumor cells (CTCs) from minimally invasive "liquid biopsy" provides critical insights into tumor biology and is critical for companion diagnostics and care. Here, we present a multiplexed spiral biochip for ultra-high throughput isolation of CTCs using inertial microfluidics to realize a single step label-free enrichment process. This device is capable of efficient cell separation of clinically relevant blood volumes in a short period of time (7.5 mL blood in 35 min). CTCs were successfully detected and isolated from 100% (50/50) blood samples collected from patients with advanced stage metastatic breast and lung cancer. They were identified under immunofluorescence assays (cytokeratin positive), as well as molecular probes (EGFR or HER2 positive). CTC recovery rate ranges from 3-1535 CTCs/mL and obtained under high purity (1 CTC for every 30-100 white blood cells detected). Retrieved cells are unlabelled and hence more viable for propagation, drug development and other downstream analysis.

Research Area(s)

  • Cancer, Circulating tumor cells, Clinical diagnostics, Inertial microfluidics, Molecular probes

Citation Format(s)

CIRCULATING TUMOR CELL (CTC) ENRICHMENT : ULTRA HIGH THROUGHPUT PROCESSING OF CLINICALLY RELEVANT BLOOD VOLUMES USING A MULTIPLEXED SPIRAL BIOCHIP. / Warkiani, M.E.; Khoo, B.L.; Tan, D.S.W. et al.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013). Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 1156-1158.

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review