Circulating miR-99b-5p as a novel predictor of erosion progression on high-resolution peripheral quantitative computed tomography in early rheumatoid arthritis : A prospective cohort study

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

2 Scopus Citations
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Author(s)

  • Jiang Yue
  • James F. Griffith
  • Jiankun Xu
  • Fan Xiao
  • Lin Shi
  • Defeng Wang
  • Priscilla C. H. Wong
  • Edmund K. Li
  • Lydia P. Tam
  • Martin Li
  • Tena K. Li
  • Wah Yan Mak
  • Vivian Hung
  • Ling Qin
  • Lai-Shan Tam

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)1724-1733
Journal / PublicationInternational Journal of Rheumatic Diseases
Volume22
Issue number9
Online published4 Jul 2019
Publication statusPublished - Sep 2019

Abstract

Objectives: To compare micro RNA (miRNA) expression: (a) between healthy individuals and early rheumatoid arthritis (ERA) patients with and without erosion on high-resolution peripheral quantitative computed tomography (HR-pQCT) at baseline; and (b) to explore whether these miRNAs could inform a signature predictive of erosion progression despite treatment with conventional synthetic disease-modifying antirheumatic drugs (csDMARDs). 
Methods: The second metacarpophalangeal head (MCP2) was scanned by HR-pQCT at baseline and 1 year in 117 ERA patients. We performed global profiling of 377 miRNAs in 10 ERA patients with and without erosion on HR-pQCT at baseline and six healthy controls. Validation of the miRNAs of interest were conducted using TaqMan® quantitative real-time polymerase chain reaction in the validation ERA cohort (n = 117) at baseline. Correlation between the candidate miRNAs and erosion progression over 1 year were also assessed. 
Results: In the 377 screened miRNAs, 94 (60.6%) miRNAs were upregulated in patients with erosions, with 13 (8.4%) upregulated more than 2-fold. Sixty-one (39.4%) miRNAs were downregulated in patients with erosions, with 6 (3.9%) downregulated more than 2-fold. Expression of miR-143-3p, miR-145-5p and miR-99b-5p were significantly higher in the plasma of ERA patients with erosions compared with those without erosions. Logistic regression analysis revealed that the baseline expression of miR-99b-5p was an independent predictor of erosion progression at month 12 (Exp [B] = 4.257, 95% CI 1.178-15.386, P = 0.027). 
Conclusions: Differential expressions of circulating miR-143-3p, miR-145-5p and miR-99b-5p in the plasma of ERA patients may characterize a severe form of the disease. MiR-99b-5p, in particular, may serve as a possible predictor for erosion progression.

Research Area(s)

  • early rheumatoid arthritis, erosion progression, HR-pQCT, miRNAs

Citation Format(s)

Circulating miR-99b-5p as a novel predictor of erosion progression on high-resolution peripheral quantitative computed tomography in early rheumatoid arthritis : A prospective cohort study. / Yue, Jiang; Lau, Terrence C. K.; Griffith, James F.; Xu, Jiankun; Xiao, Fan; Shi, Lin; Wang, Defeng; Wong, Priscilla C. H.; Li, Edmund K.; Tam, Lydia P.; Li, Martin; Li, Tena K.; Mak, Wah Yan; Hung, Vivian; Qin, Ling; Tam, Lai-Shan.

In: International Journal of Rheumatic Diseases, Vol. 22, No. 9, 09.2019, p. 1724-1733.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal