CHO cell dysfunction due to radiation-induced bystander signals observed by real-time electrical impedance measurement

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Detail(s)

Original languageEnglish
Article number113142
Journal / PublicationBiosensors and Bioelectronics
Volume181
Online published5 Mar 2021
Publication statusPublished - 1 Jun 2021

Abstract

Radiation-induced bystander effects (RIBE) have raised many concerns about radiation safety and protection. In RIBE, unirradiated cells receive signals from irradiated cells and exhibit irradiation effects. Until now, most RIBE studies have been based on morphological and biochemical characterization. However, research on the impact of RIBE on biophysical properties of cells has been lagging. Non-invasive indium tin oxide (ITO)-based impedance systems have been used as bioimpedance sensors for monitoring cell behaviors. This powerful technique has not been applied to RIBE research. In this work, we employed an electrical cell-ITO substrate impedance system (ECIIS) to study the RIBE on Chinese hamster ovary (CHO) cells. The bioimpedance of bystander CHO cells (BCHO), alpha(α)-particle (Am-241) irradiated CHO (ICHO), and untreated/unirradiated CHO (UCHO) cells were monitored with a sampling interval of 8 seconds over a period of 24 hours. Media from ICHO cells exposed to different radiation doses (0.3 nGy, 0.5 nGy, and 0.7 nGy) were used to investigate the radiation dose dependence of BCHO cells' impedance. In parallel, we imaged the cells at times where impedance changes were observed. By analyzing the changes in absolute impedance and cell size/cell number with time, we observed that BCHO cells mimicked ICHO cells in terms of modification in cell morphology and proliferation rate. Furthermore, these effects appeared to be time-dependent and inversely proportional to the radiation dose. Hence, this approach allows a label-free study of cellular responses to RIBE with high sensitivity and temporal resolution and can provide crucial insights into the RIBE mechanism.

Research Area(s)

  • Radiation-induced bystander effects, electrical cell-ITO substrate impedance system (ECIIS), Chinese hamster ovary (CHO) cells, real-time sensing

Citation Format(s)

CHO cell dysfunction due to radiation-induced bystander signals observed by real-time electrical impedance measurement. / Ilyas, A.M.; Alam, Md Kowsar; Musah, Jamal-Deen; Yang, Mengsu; Vellaisamy, A. L. Roy; Lam, Yun Wah; Lau, Condon.

In: Biosensors and Bioelectronics, Vol. 181, 113142, 01.06.2021.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review