TY - JOUR
T1 - Characterization of the ribosomal RNA methylase gene erm(A) and its promoter mutation in Campylobacter coli from chicken cecum
AU - Liu, Ting
AU - Liu, Xiao
AU - Dai, Xingyang
AU - Huang, Jinhu
AU - Li, Runsheng
AU - Wang, Liping
AU - Wang, Xiaoming
PY - 2025/4/16
Y1 - 2025/4/16
N2 - Macrolide antibiotics are commonly used to treat campylobacteriosis in both clinical settings and animal husbandry. The emergence of macrolide-resistant Campylobacter poses public health risks. In this study, six Campylobacter coli strains carrying erm(A) gene were identified from chicken cecum samples, and the functionality and genetic environment of erm(A) were analyzed. Antimicrobial susceptibility testing was conducted on Campylobacter isolates. WGS was used to analyze the genetic characteristics of erm(A)-positive strains. Cloning and in vitro-induced drug resistance assay were used to investigate the function of erm(A) gene. A total of 42 (20.2 %) C. coli isolates were obtained from 208 samples collected from chicken cecum, among which 6 erm(A)-positive. Induced drug resistance assay revealed a cytosine insertion at −54 bp/−55 bp in the erm(A) promoter, leading to a 4-fold increase in erythromycin MIC. WGS analysis showed that the erm(A) gene in C. coli shared high nucleotide sequence identity with that in Enterococcus faecalis and co-located with optrA between IS1216. To the best of our knowledge, this is a report of the prevalence and characterization of erm(A)-positive C. coli from chicken cecum. A cytosine insertion at −54 bp/−55 bp in the erm(A) promoter, leading to a 4-fold increased erythromycin MIC in Campylobacter coli. © 2025 Elsevier B.V.
AB - Macrolide antibiotics are commonly used to treat campylobacteriosis in both clinical settings and animal husbandry. The emergence of macrolide-resistant Campylobacter poses public health risks. In this study, six Campylobacter coli strains carrying erm(A) gene were identified from chicken cecum samples, and the functionality and genetic environment of erm(A) were analyzed. Antimicrobial susceptibility testing was conducted on Campylobacter isolates. WGS was used to analyze the genetic characteristics of erm(A)-positive strains. Cloning and in vitro-induced drug resistance assay were used to investigate the function of erm(A) gene. A total of 42 (20.2 %) C. coli isolates were obtained from 208 samples collected from chicken cecum, among which 6 erm(A)-positive. Induced drug resistance assay revealed a cytosine insertion at −54 bp/−55 bp in the erm(A) promoter, leading to a 4-fold increase in erythromycin MIC. WGS analysis showed that the erm(A) gene in C. coli shared high nucleotide sequence identity with that in Enterococcus faecalis and co-located with optrA between IS1216. To the best of our knowledge, this is a report of the prevalence and characterization of erm(A)-positive C. coli from chicken cecum. A cytosine insertion at −54 bp/−55 bp in the erm(A) promoter, leading to a 4-fold increased erythromycin MIC in Campylobacter coli. © 2025 Elsevier B.V.
KW - Campylobacter coli
KW - erm(A)
KW - Erythromycin resistance
KW - Site mutations
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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-86000170175&origin=recordpage
U2 - 10.1016/j.ijfoodmicro.2025.111138
DO - 10.1016/j.ijfoodmicro.2025.111138
M3 - RGC 21 - Publication in refereed journal
SN - 0168-1605
VL - 434
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
M1 - 111138
ER -