Characterization of the product ions from the collision-induced dissociation of argentinated peptides

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

70 Scopus Citations
View graph of relations

Author(s)

  • Ivan K Chu
  • Tamer Shoeib
  • Xu Guo
  • Christopher F Rodriquez
  • Alan C Hopkinson
  • And 1 others
  • Tai-Chu Lau

Detail(s)

Original languageEnglish
Pages (from-to)163-175
Journal / PublicationJournal of the American Society for Mass Spectrometry
Volume12
Issue number2
Publication statusPublished - 2001

Abstract

Tandem mass spectrometry performed on a pool of 18 oligopeptides shows that the product ion spectra of argentinated peptides, the [bn + OH + Ag]+ ions and the [yn - H + Ag]+ ions bearing identical sequences are virtually identical. These observations suggest strongly that these ions have identical structures in the gas phase. The structures of argentinated glycine, glycylglycine, and glycylglycylglycine were calculated using density functional theory (DFT) at the B3LYP/DZVP level of theory; they were independently confirmed using HF/LANL2DZ. For argentinated glycylglycylglycine, the most stable structure is one in which Ag+ is tetracoordinate and attached to the amino nitrogen and the three carbonyl oxygen atoms. Mechanisms are proposed for the fragmentation of this structure to the [b2 + OH + Ag]+ and the [y2 - H + Ag]+ ions that are consistent with all experimental observations and known calculated structures and energetics. The structures of the [b2 - H + Ag]+ and the [a2 - H + Ag]+ ions of glycylglycylglycine were also calculated using DFT. These results confirm earlier suggestions that the [b2 - H + Ag]+ ion is an argentinated oxazolone and the [a2 - H + Ag]+ an argentinated immonium ion. Copyright © 2001 American Society for Mass Spectrometry.

Citation Format(s)

Characterization of the product ions from the collision-induced dissociation of argentinated peptides. / Chu, Ivan K; Shoeib, Tamer; Guo, Xu et al.
In: Journal of the American Society for Mass Spectrometry, Vol. 12, No. 2, 2001, p. 163-175.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review