Abstract
A single-stranded S1 nuclease hypersensitive site which contains a d(AT)18 sequence structure located in the 5′-non transcription spacer of silkworm A. ricini ribosomal RNA gene has been reported[1]. Using starved-refed silkworms, another S1 nuclease sensitive site was found existing in the rDNA chromatin, while under merely starving, this S1 sensitive site disappeared[2]. Recently this inducible S1 sensitive site has been further determined. It consists of a d(GT)10--d(AT)10 special DNA sequence at the transcription initiation region, and shows a behavior of ease in DNA-unwinding, indicating that S1 nuclease sensitive sites may have an important function in the regulation of rDNA transcription and replication.
| Original language | English |
|---|---|
| Pages (from-to) | 398-405 |
| Journal | Science in China, Series C: Life Sciences |
| Volume | 40 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 1997 |
| Externally published | Yes |
Research Keywords
- DNA-unwinding element
- Ribosomal RNA gene
- S1 nuclease sensitive site
- Southern hybridization
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