Abstract
A novel porcine parvovirus designated as porcine parvovirus 2 (PPV2) was initially identified in Myanmar in 2001, in China during 2006-2007, and in Hungary in 2012. To investigate the presence and prevalence of PPV2 in the USA, a novel TaqMan® real-time PCR method was developed and used for a PPV2 survey using 483 lung samples, 185 fecal samples and 122 sera collected from pigs on 295 farms in 18 U.S. states. The overall prevalence of PPV2 was 20.7% (100/483) in lung samples and 7.6% (14/185) in fecal samples obtained from pigs of different age groups, and 1.6% (2/122) in sera or thoracic fluids obtained from neonatal pigs. Further genomic sequence comparison demonstrated that the 2011 U.S. PPV2 sequences have nucleotide identities of 95.4-97.7% with the 2006-2007 strains detected in China while the nucleotide identity was 94.7% with the 2001 strain detected in Myanmar, indicating persistent evolution of the virus. Phylogenetic and sequence homology analyses demonstrated a close relationship of PPV2 with members of the proposed genus PARV4-like virus, and the classification of PPV2 into this proposed genus is suggested. © 2012 Elsevier B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 325-330 |
| Journal | Veterinary Microbiology |
| Volume | 161 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - 25 Jan 2013 |
| Externally published | Yes |
Bibliographical note
Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]Research Keywords
- Pigs
- Porcine parvovirus type 2
- PPV2
- Prevalence
- USA