Characterization of glucose isomerase-producing bacteria and optimization of fermentation conditions for producing glucose isomerase using biomass
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 239-249 |
Journal / Publication | Green Chemical Engineering |
Volume | 4 |
Issue number | 2 |
Online published | 21 May 2022 |
Publication status | Published - Jun 2023 |
Externally published | Yes |
Link(s)
DOI | DOI |
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Attachment(s) | Documents
Publisher's Copyright Statement
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85131674796&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(d3c9cabb-0727-40bb-a639-12382b722f71).html |
Abstract
Glucose isomerase (GI) is an enzyme with high potential applications. Characterization of GI producing bacteria with interesting properties from an industrial point of view is essential. Bacillus sp., Paenarthrobacter sp., Chryseobacterium sp., Hymenobacter sp., Mycobacterium sp., and Stenotrophomonas sp. were isolated from soil samples. Optimization of enzyme production yield was investigated in various fermentation conditions using response surface methodology. All isolates exhibited maximum GI activity at 40 °C, pH 6–8 after 4 days of incubation. A mixture of peptone/yeast extract or tryptone/peptone enhanced higher enzyme production. The same trend was observed in fermentation medium containing 1% xylose or 2%–2.5% wheat straw. This study advanced the knowledge of these bacterial isolates in promoting wheat straw as feedstock for the bio-based industry. © 2022 Institute of Process Engineering, Chinese Academy of Sciences.
Research Area(s)
- 16S rRNA, Biomass conversion, Cellulolytic bacteria, Glucose/xylose isomerase
Citation Format(s)
Characterization of glucose isomerase-producing bacteria and optimization of fermentation conditions for producing glucose isomerase using biomass. / Mokale Kognou, Aristide Laurel; Chio, Chonlong; Khatiwada, Janak Raj et al.
In: Green Chemical Engineering, Vol. 4, No. 2, 06.2023, p. 239-249.
In: Green Chemical Engineering, Vol. 4, No. 2, 06.2023, p. 239-249.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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