Characterization of glucose isomerase-producing bacteria and optimization of fermentation conditions for producing glucose isomerase using biomass

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

5 Scopus Citations
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Author(s)

  • Aristide Laurel Mokale Kognou
  • Chonlong Chio
  • Janak Raj Khatiwada
  • Sarita Shrestha
  • Xuantong Chen
  • Hongwei Li
  • Yuen Zhu
  • Zi-Hua Jiang
  • Wensheng Qin

Detail(s)

Original languageEnglish
Pages (from-to)239-249
Journal / PublicationGreen Chemical Engineering
Volume4
Issue number2
Online published21 May 2022
Publication statusPublished - Jun 2023
Externally publishedYes

Link(s)

Abstract

Glucose isomerase (GI) is an enzyme with high potential applications. Characterization of GI producing bacteria with interesting properties from an industrial point of view is essential. Bacillus sp., Paenarthrobacter sp., Chryseobacterium sp., Hymenobacter sp., Mycobacterium sp., and Stenotrophomonas sp. were isolated from soil samples. Optimization of enzyme production yield was investigated in various fermentation conditions using response surface methodology. All isolates exhibited maximum GI activity at 40 °C, pH 6–8 after 4 days of incubation. A mixture of peptone/yeast extract or tryptone/peptone enhanced higher enzyme production. The same trend was observed in fermentation medium containing 1% xylose or 2%–2.5% wheat straw. This study advanced the knowledge of these bacterial isolates in promoting wheat straw as feedstock for the bio-based industry. © 2022 Institute of Process Engineering, Chinese Academy of Sciences.

Research Area(s)

  • 16S rRNA, Biomass conversion, Cellulolytic bacteria, Glucose/xylose isomerase

Citation Format(s)

Characterization of glucose isomerase-producing bacteria and optimization of fermentation conditions for producing glucose isomerase using biomass. / Mokale Kognou, Aristide Laurel; Chio, Chonlong; Khatiwada, Janak Raj et al.
In: Green Chemical Engineering, Vol. 4, No. 2, 06.2023, p. 239-249.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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