Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad

Dal Y. Kim; Hilda Guzman; Rudy Bueno Jr.; James A. Dennett; Albert J. Auguste; Christine V.F. Carrington; Vsevolod L. Popov; Scott C. Weaver; David W.C. Beasley; Robert B. Tesh

doi:10.1016/j.virol.2008.12.034

Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad

Dal Y. Kim, Hilda Guzman, Rudy Bueno Jr., James A. Dennett, Albert J. Auguste, Christine V.F. Carrington, Vsevolod L. Popov, Scott C. Weaver, David W.C. Beasley, Robert B. Tesh^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

94 Citations (Scopus)

Abstract

Recent reports indicate that flaviviruses similar to the cell fusing agent virus (CFAV) naturally infect a wide variety of mosquito species. These newly recognized insect-specific viruses comprise a distinct CFAV complex within the genus Flavivirus. Here, we describe the isolation and characterization of nine strains of Culex flavivirus (Cx FV), a member of the CFAV complex, from mosquitoes collected in the United States (East Texas) and Trinidad. Phylogenetic analyses of the envelope protein gene sequences of these nine mosquito isolates with those of other CFAV complex flaviviruses in GenBank indicate that the U.S. isolates group with CxFV isolates from Asia (Japan and Indonesia), while the Trinidad isolates are more similar to CxFV isolates from Central America. A discussion follows on the possible biological significance of the CFAV complex flaviviruses.

Original language	English
Pages (from-to)	154-159
Number of pages	6
Journal	Virology
Volume	386
Issue number	1
Online published	3 Feb 2009
DOIs	https://doi.org/10.1016/j.virol.2008.12.034
Publication status	Published - 30 Mar 2009
Externally published	Yes

Research Keywords

Animals
Cell Line
Cluster Analysis
Culex/virology
Flavivirus/genetics
Gene Order
Gene Products, env/genetics
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA
Sequence Homology
Texas
Trinidad and Tobago

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title = "Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad",

abstract = "Recent reports indicate that flaviviruses similar to the cell fusing agent virus (CFAV) naturally infect a wide variety of mosquito species. These newly recognized insect-specific viruses comprise a distinct CFAV complex within the genus Flavivirus. Here, we describe the isolation and characterization of nine strains of Culex flavivirus (Cx FV), a member of the CFAV complex, from mosquitoes collected in the United States (East Texas) and Trinidad. Phylogenetic analyses of the envelope protein gene sequences of these nine mosquito isolates with those of other CFAV complex flaviviruses in GenBank indicate that the U.S. isolates group with CxFV isolates from Asia (Japan and Indonesia), while the Trinidad isolates are more similar to CxFV isolates from Central America. A discussion follows on the possible biological significance of the CFAV complex flaviviruses.",

keywords = "Animals, Cell Line, Cluster Analysis, Culex/virology, Flavivirus/genetics, Gene Order, Gene Products, env/genetics, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Sequence Homology, Texas, Trinidad and Tobago",

author = "Kim, {Dal Y.} and Hilda Guzman and {Bueno Jr.}, Rudy and Dennett, {James A.} and Auguste, {Albert J.} and Carrington, {Christine V.F.} and Popov, {Vsevolod L.} and Weaver, {Scott C.} and Beasley, {David W.C.} and Tesh, {Robert B.}",

year = "2009",

month = mar,

day = "30",

doi = "10.1016/j.virol.2008.12.034",

language = "English",

volume = "386",

pages = "154--159",

journal = "Virology",

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TY - JOUR

T1 - Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad

AU - Kim, Dal Y.

AU - Guzman, Hilda

AU - Bueno Jr., Rudy

AU - Dennett, James A.

AU - Auguste, Albert J.

AU - Carrington, Christine V.F.

AU - Popov, Vsevolod L.

AU - Weaver, Scott C.

AU - Beasley, David W.C.

AU - Tesh, Robert B.

PY - 2009/3/30

Y1 - 2009/3/30

N2 - Recent reports indicate that flaviviruses similar to the cell fusing agent virus (CFAV) naturally infect a wide variety of mosquito species. These newly recognized insect-specific viruses comprise a distinct CFAV complex within the genus Flavivirus. Here, we describe the isolation and characterization of nine strains of Culex flavivirus (Cx FV), a member of the CFAV complex, from mosquitoes collected in the United States (East Texas) and Trinidad. Phylogenetic analyses of the envelope protein gene sequences of these nine mosquito isolates with those of other CFAV complex flaviviruses in GenBank indicate that the U.S. isolates group with CxFV isolates from Asia (Japan and Indonesia), while the Trinidad isolates are more similar to CxFV isolates from Central America. A discussion follows on the possible biological significance of the CFAV complex flaviviruses.

AB - Recent reports indicate that flaviviruses similar to the cell fusing agent virus (CFAV) naturally infect a wide variety of mosquito species. These newly recognized insect-specific viruses comprise a distinct CFAV complex within the genus Flavivirus. Here, we describe the isolation and characterization of nine strains of Culex flavivirus (Cx FV), a member of the CFAV complex, from mosquitoes collected in the United States (East Texas) and Trinidad. Phylogenetic analyses of the envelope protein gene sequences of these nine mosquito isolates with those of other CFAV complex flaviviruses in GenBank indicate that the U.S. isolates group with CxFV isolates from Asia (Japan and Indonesia), while the Trinidad isolates are more similar to CxFV isolates from Central America. A discussion follows on the possible biological significance of the CFAV complex flaviviruses.

KW - Animals

KW - Cell Line

KW - Cluster Analysis

KW - Culex/virology

KW - Flavivirus/genetics

KW - Gene Order

KW - Gene Products, env/genetics

KW - Molecular Sequence Data

KW - Phylogeny

KW - Sequence Analysis, DNA

KW - Sequence Homology

KW - Texas

KW - Trinidad and Tobago

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U2 - 10.1016/j.virol.2008.12.034

DO - 10.1016/j.virol.2008.12.034

M3 - RGC 21 - Publication in refereed journal

C2 - 19193389

SN - 0042-6822

VL - 386

SP - 154

EP - 159

JO - Virology

JF - Virology

IS - 1

ER -

Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad

Abstract

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