Characterization of a regulatory region in the N-terminus of rabbit Kv1.3

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Xiaoqiang Yao
  • Yu Huang
  • Hiu-Yee Kwan
  • Pat Chan
  • Gary Desir

Detail(s)

Original languageEnglish
Pages (from-to)492-498
Journal / PublicationBiochemical and Biophysical Research Communications
Volume249
Issue number2
Publication statusPublished - 19 Aug 1998
Externally publishedYes

Abstract

The N-terminus of rabbit Kv1.3 contains a signal sequence which regulates expression of Kv1.3 proteins in the plasma membrane. Removal of an N-terminal region (aa3-39) produced an increase in expressed K+ current. Progressive deletion at the N-terminus demonstrated that the shortest deletion required for the elevation of K current is D6-34. Since the functional signal sequence must include both ends of the peptide segment aa6-34 where charged residues are densely distributed, it is conceivable that this N-terminal signal sequence is related to charge or its associated hydrophilicity. Removal of two charged residues (31R, 33E) through amino acid substitution which converts deletion construct D3-27 to D3-27* effectively raises the amplitude of expressed current, further indicating the importance of charged residues. With the use of a mutated dynamin and a soluble N-terminal peptide, we also revealed that the N-terminal signal sequence may not act through the endocytotic pathway.

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Citation Format(s)

Characterization of a regulatory region in the N-terminus of rabbit Kv1.3. / Yao, Xiaoqiang; Huang, Yu; Kwan, Hiu-Yee; Chan, Pat; Desir, Gary.

In: Biochemical and Biophysical Research Communications, Vol. 249, No. 2, 19.08.1998, p. 492-498.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review