Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N

Ming-Chang Chan; Shih-Hsin Ho; Duu-Jong Lee; Chun-Yen Chen; Chieh-Chen Huang; Jo-Shu Chang

doi:10.1016/j.bej.2012.11.017

Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N

Ming-Chang Chan, Shih-Hsin Ho, Duu-Jong Lee, Chun-Yen Chen, Chieh-Chen Huang, Jo-Shu Chang

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

109 Citations (Scopus)

Abstract

This study aimed to improve the commercial viability of microalgae-based lutein production using an isolated microalga Scenedesmus obliquus CNW-N possessing a high lutein content of over 0.25%. Effective lutein extraction protocols, appropriate storage methods, and purification procedures were developed. Disruption of microalgae cells was most efficient with a bead-beater. The conventional saponification step was modified to reduce the overall extraction time by 24. h. Diethyl ether exhibited the best lutein extraction efficiency. Storage of the lutein extract at low temperature (4 or -20. °C) with antioxidant addition (around 0.01% BHT) can maintain 90% lutein stability after 80 days. Addition of a suitable amount of the antioxidant could promote the stability of lutein extracts under the exposure of light. The protocol developed in this work allows efficient lutein extraction from S. obliquus CNW-N at a lower cost. Further purification was employed to elevate the purity of lutein and its commercial value. © 2012 Elsevier B.V.

Original language	English
Pages (from-to)	24-31
Journal	Biochemical Engineering Journal
Volume	78
DOIs	https://doi.org/10.1016/j.bej.2012.11.017
Publication status	Published - 5 Sept 2013
Externally published	Yes

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Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Research Keywords

Bioseparations
Extraction kinetics
Lutein
Microalgae
Purification
Scenedesmus obliquus

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title = "Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N",

abstract = "This study aimed to improve the commercial viability of microalgae-based lutein production using an isolated microalga Scenedesmus obliquus CNW-N possessing a high lutein content of over 0.25%. Effective lutein extraction protocols, appropriate storage methods, and purification procedures were developed. Disruption of microalgae cells was most efficient with a bead-beater. The conventional saponification step was modified to reduce the overall extraction time by 24. h. Diethyl ether exhibited the best lutein extraction efficiency. Storage of the lutein extract at low temperature (4 or -20. °C) with antioxidant addition (around 0.01% BHT) can maintain 90% lutein stability after 80 days. Addition of a suitable amount of the antioxidant could promote the stability of lutein extracts under the exposure of light. The protocol developed in this work allows efficient lutein extraction from S. obliquus CNW-N at a lower cost. Further purification was employed to elevate the purity of lutein and its commercial value. {\textcopyright} 2012 Elsevier B.V.",

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T1 - Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N

AU - Chan, Ming-Chang

AU - Ho, Shih-Hsin

AU - Lee, Duu-Jong

AU - Chen, Chun-Yen

AU - Huang, Chieh-Chen

AU - Chang, Jo-Shu

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N2 - This study aimed to improve the commercial viability of microalgae-based lutein production using an isolated microalga Scenedesmus obliquus CNW-N possessing a high lutein content of over 0.25%. Effective lutein extraction protocols, appropriate storage methods, and purification procedures were developed. Disruption of microalgae cells was most efficient with a bead-beater. The conventional saponification step was modified to reduce the overall extraction time by 24. h. Diethyl ether exhibited the best lutein extraction efficiency. Storage of the lutein extract at low temperature (4 or -20. °C) with antioxidant addition (around 0.01% BHT) can maintain 90% lutein stability after 80 days. Addition of a suitable amount of the antioxidant could promote the stability of lutein extracts under the exposure of light. The protocol developed in this work allows efficient lutein extraction from S. obliquus CNW-N at a lower cost. Further purification was employed to elevate the purity of lutein and its commercial value. © 2012 Elsevier B.V.

AB - This study aimed to improve the commercial viability of microalgae-based lutein production using an isolated microalga Scenedesmus obliquus CNW-N possessing a high lutein content of over 0.25%. Effective lutein extraction protocols, appropriate storage methods, and purification procedures were developed. Disruption of microalgae cells was most efficient with a bead-beater. The conventional saponification step was modified to reduce the overall extraction time by 24. h. Diethyl ether exhibited the best lutein extraction efficiency. Storage of the lutein extract at low temperature (4 or -20. °C) with antioxidant addition (around 0.01% BHT) can maintain 90% lutein stability after 80 days. Addition of a suitable amount of the antioxidant could promote the stability of lutein extracts under the exposure of light. The protocol developed in this work allows efficient lutein extraction from S. obliquus CNW-N at a lower cost. Further purification was employed to elevate the purity of lutein and its commercial value. © 2012 Elsevier B.V.

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KW - Extraction kinetics

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KW - Microalgae

KW - Purification

KW - Scenedesmus obliquus

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DO - 10.1016/j.bej.2012.11.017

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JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

ER -

Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N

Abstract

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Research Keywords

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