Changes of Adipose Tissue Morphology and Composition during Late Pregnancy and Early Lactation in Dairy Cows

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

11 Scopus Citations
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Author(s)

  • Anna Kulcsár
  • Franziska Kluge
  • Idir Benbelkacem
  • Kathrin Hansen
  • Lena Locher
  • Ulrich Meyer
  • Jürgen Rehage
  • Sven Dänicke
  • Korinna Huber

Detail(s)

Original languageEnglish
Article numbere0127208
Journal / PublicationPLoS ONE
Volume10
Issue number5
Publication statusPublished - 15 May 2015
Externally publishedYes

Abstract

Dairy cows mobilize large amounts of body fat during early lactation to overcome negative energy balance which typically arises in this period. As an adaptation process, adipose tissues of cows undergo extensive remodeling during late pregnancy and early lactation. The objective of the present study was to characterize this remodeling to get a better understanding of adaptation processes in adipose tissues, affected by changing metabolic conditions including lipid mobilization and refilling as a function of energy status. This was done by determining adipocyte size in histological sections of subcutaneous and retroperitoneal adipose tissue biopsy samples collected from German Holstein cows at 42 days prepartum, and 1, 21, and 100 days postpartum. Characterization of cell size changes was extended by the analysis of DNA, triacylglycerol, and protein content per gram tissue, and β-actin protein expression in the same samples. In both adipose tissue depots cell size was becoming smaller during the course of the study, suggesting a decrease in cellular triacylglycerol content. Results of DNA, triacylglycerol, and protein content, and β-actin protein expression could only partially explain the observed differences in cell size. The retroperitoneal adipose tissue exhibited a greater extent of time-related differences in cell size, DNA, and protein content, suggesting greater dynamics and metabolic flexibility for this abdominal depot compared to the investigated subcutaneous depot.

Research Area(s)

  • Adipose tissue, Adipocytes, Lactation, Lipids, Biopsy, Lipid metabolism, Protein expression, Histology

Citation Format(s)

Changes of Adipose Tissue Morphology and Composition during Late Pregnancy and Early Lactation in Dairy Cows. / Kenéz, Ákos; Kulcsár, Anna; Kluge, Franziska; Benbelkacem, Idir; Hansen, Kathrin; Locher, Lena; Meyer, Ulrich; Rehage, Jürgen; Dänicke, Sven; Huber, Korinna.

In: PLoS ONE, Vol. 10, No. 5, e0127208, 15.05.2015.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal