Cellular uptake and activity of heparin functionalised cerium oxide nanoparticles in monocytes

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • S.R. Simon Ting
  • John M. Whitelock
  • Romana Tomic
  • Cindy Gunawan
  • Rose Amal
  • Megan S. Lord

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)4377-4386
Journal / PublicationBiomaterials
Volume34
Issue number17
Publication statusPublished - Jun 2013

Abstract

Cerium oxide nanoparticles (nanoceria) are effective in scavenging intracellular reactive oxygen species (ROS). In this study nanoceria synthesized by flame spray pyrolysis (dXRD = 12 nm) were functionalised with heparin via an organosilane linker, 3-aminopropyltriethoxysilane. Nanoceria were functionalised with approximately 130 heparin molecules per nanoparticle as determined by thermo gravimetric analysis. Heparin functionalised nanoceria were more effectively internalised by the human monocyte cell line, U937, and U937 cells that had been activated with phorbol 12 myristate 13-acetate (PMA) than bare nanoceria. The heparin functionalised nanoceria were also more effective in scavenging ROS than nanoceria in both activated and unactivated U937 cells. Heparin coupled nanoceria were found to be biologically active due to their ability to bind fibroblast growth factor 2 and signal through FGF receptor 1. Additionally, the heparin-coupled nanoceria, once internalised by the cells, were found to be degraded by 48 h. Together these data demonstrated that heparin enhanced the biological properties of nanoceria in terms of cellular uptake and ROS scavenging, while the nanoceria themselves were more effective at delivering heparin intracellularly than exposing cells to heparin in solution. © 2013.

Research Area(s)

  • Cerium oxide, Heparin, Macrophage, Nanoparticle, Reactive oxygen species

Citation Format(s)

Cellular uptake and activity of heparin functionalised cerium oxide nanoparticles in monocytes. / Ting, S.R. Simon; Whitelock, John M.; Tomic, Romana; Gunawan, Cindy; Teoh, Wey Yang; Amal, Rose; Lord, Megan S.

In: Biomaterials, Vol. 34, No. 17, 06.2013, p. 4377-4386.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review