TY - JOUR
T1 - Cellular level robotic surgery
T2 - Nanodissection of intermediate filaments in live keratinocytes
AU - Yang, Ruiguo
AU - Song, Bo
AU - Sun, Zhiyong
AU - Lai, King Wai Chiu
AU - Fung, Carmen Kar Man
AU - Patterson, Kevin C.
AU - Seiffert-Sinha, Kristina
AU - Sinha, Animesh A.
AU - Xi, Ning
PY - 2015/1
Y1 - 2015/1
N2 - We present the nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis and mimic the effect of pathophysiological modulation of intercellular adhesion. Nanosurgery successfully severs the intermediate filament bundles and disrupts cell-cell adhesion similar to the desmosomal protein disassembly in autoimmune disease, or the cationic modulation of desmosome formation. Our nanomechanical analysis revealed that adhesion loss results in a decrease in cellular stiffness in both cases of biochemical modulation of the desmosome junctions and mechanical disruption of intercellular adhesion, supporting the notion that intercellular adhesion through intermediate filaments anchors the cell structure as focal adhesion does and that intermediate filaments are integral components in cell mechanical integrity. The surgical process could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis. From the Clinical Editor: This team of authors performed nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis, and mimic the effect of pathophysiological modulation of intercellular adhesions. This method could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis.
AB - We present the nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis and mimic the effect of pathophysiological modulation of intercellular adhesion. Nanosurgery successfully severs the intermediate filament bundles and disrupts cell-cell adhesion similar to the desmosomal protein disassembly in autoimmune disease, or the cationic modulation of desmosome formation. Our nanomechanical analysis revealed that adhesion loss results in a decrease in cellular stiffness in both cases of biochemical modulation of the desmosome junctions and mechanical disruption of intercellular adhesion, supporting the notion that intercellular adhesion through intermediate filaments anchors the cell structure as focal adhesion does and that intermediate filaments are integral components in cell mechanical integrity. The surgical process could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis. From the Clinical Editor: This team of authors performed nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis, and mimic the effect of pathophysiological modulation of intercellular adhesions. This method could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis.
KW - Atomic Force Microscopy
KW - Cell-cell adhesion
KW - Desmosome
KW - Intermediate filament
KW - Mechanical property
KW - Nanosurgery
UR - http://www.scopus.com/inward/record.url?scp=84919620748&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84919620748&origin=recordpage
U2 - 10.1016/j.nano.2014.08.008
DO - 10.1016/j.nano.2014.08.008
M3 - RGC 21 - Publication in refereed journal
C2 - 25200612
SN - 1549-9634
VL - 11
SP - 137
EP - 145
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
IS - 1
ER -