Cell-penetration efficiency of PEGylated multi-walled carbon nanotubes is dependent on cell types

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

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Author(s)

Detail(s)

Original languageEnglish
Title of host publication2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
Pages203-208
Publication statusPublished - 2010

Conference

Title4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010)
PlaceChina
CityHong Kong/Macau
Period5 - 9 December 2010

Abstract

Carbon nanotubes (CNTs) have been widely investigated as one of the most promising nanomaterials in biomedical applications. Functionalization of CNTs with poly(ethylene glycol) diamine (PEG) is a recognized methodology with good solubility and biocompatibility. In this study, PEG conjugated (PEGylated) multi-walled CNTs (MWCNTs) were prepared and labeled with fluorophore FITC. Using fluorophore-conjugated PEGylated MWCNTs, we monitored their accumulation in transformed cancer cells and in normal cells. The intracellular accumulation of PEGylated MWCNTs was studied under confocal microscope and transmission electron microscope. At the same concentration and exposure time, PEGylated MWCNTs entered all three of the cancer cell lines tested much more efficiently than the three primary human fibroblast lines. This different cell-penetration efficiency was observed both in separate cell culture separate exposure conditions and in co-culture and co-exposure conditions. This study highlights that the cell-penetration efficiency of PEGylated MWCNTs is dependent on cell types, and possibly due to different metabolic rates in different cell types. Furthermore, the intracellular accumulation of PEGylated MWCNTs did not impair membrane integrity, and the treated cells remained normal morphology, indicating good biocompatibility of PEGylated MWCNTs. This study suggests that PEGylated MWCNTs can be developed as potential drug carrier with its intrinsic higher preference to tumor cells than normal cells. © 2010 IEEE.

Research Area(s)

  • cancer cell, carbon nanotubes, cell penetration, efficiency, normal cell

Citation Format(s)

Cell-penetration efficiency of PEGylated multi-walled carbon nanotubes is dependent on cell types. / Cheng, Jinping; Lam, Yun Wah; Sun, Ya-Ping; Cheng, Shuk Han.

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010. 2010. p. 203-208 5749835.

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review