Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

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Author(s)

  • Pan Li
  • Lianqing Liu
  • Yuzhao Zhang
  • Haibo Yu
  • Gwo-Bin Lee
  • Yuechao Wang

Related Research Unit(s)

Detail(s)

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
PublisherChemical and Biological Microsystems Society
Pages252-255
Volume1
ISBN (Print)9781510897571
Publication statusPublished - Nov 2018

Publication series

NameInternational Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

Conference

Title22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
LocationKaohsiung Exhibition Center
PlaceTaiwan
CityKaohsiung
Period11 - 15 November 2018

Abstract

Three-dimensional (3D) micro-engineered hydrogel biomaterials incorporating cells is a promising in vitro bio-model for tissue engineering. We report a visible-light induced layer-by-layer microfabrication method for large scale tunable 3D hydrogel network and its application in 3D cell culturing and modulation. This optically induced electropolymerization method was previously reported for two-dimensional (2D) hydrogel patterning and cell patterning. Here, we improved the fabrication process by designing a series of switching “digitally-controlled photomasks” to continuously fabricate multi-layered 3D net-like hydrogel scaffolds with tunable pores and gaps. Cell spreading and migrating behaviors were further investigated after incorporating them into this biocompatible hydrogel scaffold. This method may shine some light on the in vitro reconstruction of physiologically related bio-microenvironments.

Research Area(s)

  • 3D cell culture, 3D hydrogel, Bio-fabrication, Optically-induced electrokinetics

Bibliographic Note

Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).

Citation Format(s)

Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip. / Li, Pan; Liu, Lianqing; Zhang, Yuzhao; Yu, Haibo; Lee, Gwo-Bin; Wang, Yuechao; Li, Wen Jung.

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) . Vol. 1 Chemical and Biological Microsystems Society, 2018. p. 252-255 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS ).

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review