Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip

Pan Li; Lianqing Liu; Yuzhao Zhang; Haibo Yu; Gwo-Bin Lee; Yuechao Wang; Wen Jung Li

Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip

Pan Li
, Lianqing Liu
, Yuzhao Zhang
, Haibo Yu
, Gwo-Bin Lee
, Yuechao Wang
, Wen Jung Li^*

^*Corresponding author for this work

Department of Mechanical Engineering

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Three-dimensional (3D) micro-engineered hydrogel biomaterials incorporating cells is a promising in vitro bio-model for tissue engineering. We report a visible-light induced layer-by-layer microfabrication method for large scale tunable 3D hydrogel network and its application in 3D cell culturing and modulation. This optically induced electropolymerization method was previously reported for two-dimensional (2D) hydrogel patterning and cell patterning. Here, we improved the fabrication process by designing a series of switching “digitally-controlled photomasks” to continuously fabricate multi-layered 3D net-like hydrogel scaffolds with tunable pores and gaps. Cell spreading and migrating behaviors were further investigated after incorporating them into this biocompatible hydrogel scaffold. This method may shine some light on the in vitro reconstruction of physiologically related bio-microenvironments.

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
Publisher	Chemical and Biological Microsystems Society
Pages	252-255
Volume	1
ISBN (Print)	9781510897571
Publication status	Published - Nov 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, China Duration: 11 Nov 2018 → 15 Nov 2018 https://cbmsociety.org/microtas/microtas2018/ https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf https://archive.cbmsociety.org/utas/uTAS2018.zip

Publication series

Name	International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
Abbreviated title	μTAS2018
Place	Taiwan, China
City	Kaohsiung
Period	11/11/18 → 15/11/18
Internet address	https://cbmsociety.org/microtas/microtas2018/ https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf https://archive.cbmsociety.org/utas/uTAS2018.zip

Bibliographical note

Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 9 Industry, Innovation, and Infrastructure

Research Keywords

3D cell culture
3D hydrogel
Bio-fabrication
Optically-induced electrokinetics

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

Li, P., Liu, L., Zhang, Y., Yu, H., Lee, G.-B., Wang, Y., & Li, W. J. (2018). Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) (Vol. 1, pp. 252-255). (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS ). Chemical and Biological Microsystems Society.

Li, Pan ; Liu, Lianqing ; Zhang, Yuzhao et al. / Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) . Vol. 1 Chemical and Biological Microsystems Society, 2018. pp. 252-255 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS ).

@inproceedings{3a466170095d4c92b29b5307de166022,

title = "Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip",

abstract = "Three-dimensional (3D) micro-engineered hydrogel biomaterials incorporating cells is a promising in vitro bio-model for tissue engineering. We report a visible-light induced layer-by-layer microfabrication method for large scale tunable 3D hydrogel network and its application in 3D cell culturing and modulation. This optically induced electropolymerization method was previously reported for two-dimensional (2D) hydrogel patterning and cell patterning. Here, we improved the fabrication process by designing a series of switching “digitally-controlled photomasks” to continuously fabricate multi-layered 3D net-like hydrogel scaffolds with tunable pores and gaps. Cell spreading and migrating behaviors were further investigated after incorporating them into this biocompatible hydrogel scaffold. This method may shine some light on the in vitro reconstruction of physiologically related bio-microenvironments.",

keywords = "3D cell culture, 3D hydrogel, Bio-fabrication, Optically-induced electrokinetics",

author = "Pan Li and Lianqing Liu and Yuzhao Zhang and Haibo Yu and Gwo-Bin Lee and Yuechao Wang and Li, \{Wen Jung\}",

note = "Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).; 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), μTAS2018 ; Conference date: 11-11-2018 Through 15-11-2018",

year = "2018",

month = nov,

language = "English",

isbn = "9781510897571",

volume = "1",

series = "International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS ",

publisher = "Chemical and Biological Microsystems Society",

pages = "252--255",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)",

url = "https://cbmsociety.org/microtas/microtas2018/, https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf, https://archive.cbmsociety.org/utas/uTAS2018.zip",

}

Li, P, Liu, L, Zhang, Y, Yu, H, Lee, G-B, Wang, Y & Li, WJ 2018, Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) . vol. 1, International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS , Chemical and Biological Microsystems Society, pp. 252-255, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), Kaohsiung, Taiwan, China, 11/11/18.

Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip. / Li, Pan; Liu, Lianqing; Zhang, Yuzhao et al.
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) . Vol. 1 Chemical and Biological Microsystems Society, 2018. p. 252-255 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS ).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip

AU - Li, Pan

AU - Liu, Lianqing

AU - Zhang, Yuzhao

AU - Yu, Haibo

AU - Lee, Gwo-Bin

AU - Wang, Yuechao

AU - Li, Wen Jung

N1 - Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).

PY - 2018/11

Y1 - 2018/11

N2 - Three-dimensional (3D) micro-engineered hydrogel biomaterials incorporating cells is a promising in vitro bio-model for tissue engineering. We report a visible-light induced layer-by-layer microfabrication method for large scale tunable 3D hydrogel network and its application in 3D cell culturing and modulation. This optically induced electropolymerization method was previously reported for two-dimensional (2D) hydrogel patterning and cell patterning. Here, we improved the fabrication process by designing a series of switching “digitally-controlled photomasks” to continuously fabricate multi-layered 3D net-like hydrogel scaffolds with tunable pores and gaps. Cell spreading and migrating behaviors were further investigated after incorporating them into this biocompatible hydrogel scaffold. This method may shine some light on the in vitro reconstruction of physiologically related bio-microenvironments.

AB - Three-dimensional (3D) micro-engineered hydrogel biomaterials incorporating cells is a promising in vitro bio-model for tissue engineering. We report a visible-light induced layer-by-layer microfabrication method for large scale tunable 3D hydrogel network and its application in 3D cell culturing and modulation. This optically induced electropolymerization method was previously reported for two-dimensional (2D) hydrogel patterning and cell patterning. Here, we improved the fabrication process by designing a series of switching “digitally-controlled photomasks” to continuously fabricate multi-layered 3D net-like hydrogel scaffolds with tunable pores and gaps. Cell spreading and migrating behaviors were further investigated after incorporating them into this biocompatible hydrogel scaffold. This method may shine some light on the in vitro reconstruction of physiologically related bio-microenvironments.

KW - 3D cell culture

KW - 3D hydrogel

KW - Bio-fabrication

KW - Optically-induced electrokinetics

UR - https://www.scopus.com/pages/publications/85079895407

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85079895407&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781510897571

VL - 1

T3 - International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

SP - 252

EP - 255

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)

Y2 - 11 November 2018 through 15 November 2018

ER -

Li P, Liu L, Zhang Y, Yu H, Lee GB, Wang Y et al. Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) . Vol. 1. Chemical and Biological Microsystems Society. 2018. p. 252-255. (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS ).

Cell Culturing in Electropolymerized Hydrogel Multi-Layer Nets Fabricated in an Electrokinetics Microfluidic Chip

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Research Keywords

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this