Cell Chiral Orientation Enhanced by Intercellular Alignment

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

1 Scopus Citations
View graph of relations

Detail(s)

Original languageEnglish
Title of host publicationProceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering
PublisherIEEE
Pages104-108
ISBN (electronic)978-1-5386-7579-3
Publication statusPublished - Dec 2018

Publication series

NameIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
Volume2018-December
ISSN (Print)2159-6964
ISSN (electronic)2159-6972

Conference

Title12th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2018)
PlaceUnited States
CityWaikiki Beach
Period2 - 5 December 2018

Abstract

Cells exhibit chiral orientation on micropatterned stripes, which reflects the development of left-right (LR) asymmetry in tissue microarchitecture. We previously reported a dependence of cell chirality on substrate stiffness, as more cells oriented with negative angles on glass but not on PDMS substrate. However, the mechanism remains unclear. Here, using an image processing method which automates segmentation of individual nucleus, we found that enhanced chirality correlates with an increased intercellular alignment on glass substrate. More interestingly, comparing image with low cell density (< 50,000 cells/cm2 ) to high cell density (> 180,000 cells/cm2 ), the increased cell density does not improve the intercellular alignment but only reduces the distance between neighbouring cells, making the chirality independent of density range of tests.

Citation Format(s)

Cell Chiral Orientation Enhanced by Intercellular Alignment. / Kwong, Hoi Kwan; Huang, Yaozhun; Bao, Yuanye et al.
Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering. IEEE, 2018. p. 104-108 8641654 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED; Vol. 2018-December).

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review