Cell behaviors mediated by the scale of extracellular matrix micro-islands

Shuhuan Hu; Raymond H.W. Lam

doi:10.1109/NANOMED.2016.7883489

Cell behaviors mediated by the scale of extracellular matrix micro-islands

Shuhuan Hu
, Raymond H.W. Lam^*

^*Corresponding author for this work

CityUHK Shenzhen Research Institute

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Extracellular matrix (ECM) plays a critical role in regulating cell behaviors such as proliferation, spreading, migration and differentiation. Here we investigate ECM micro-patterns as a factor of cell-ECM interactions. Different sizes of circular ECM islands were fabricated using the microcontact printing (μCP) technique as an array of cell attachment sites spreading over the substrates. We investigate the scale of ECM islands on their effects on cell morphology and the focal adhesions (FAs) characteristics. Further, we observe morphological changes and cytoskeleton formation during the cell spreading stage of cells on different ECM patterns, revealing that the size and gap distance between the patterns influences the cell spreading as well as the dynamic cytoskeleton architecture significantly.

Original language	English
Title of host publication	IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
Publisher	IEEE Computer Society
Pages	65-68
ISBN (Print)	9781509046751
DOIs	https://doi.org/10.1109/NANOMED.2016.7883489
Publication status	Published - 20 Mar 2017
Event	10th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2016 - Macau, China Duration: 30 Oct 2016 → 2 Nov 2016

Publication series

Name
ISSN (Print)	2159-6964
ISSN (Electronic)	2159-6972

Conference

Conference	10th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2016
Place	China
City	Macau
Period	30/10/16 → 2/11/16

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10.1109/NANOMED.2016.7883489

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@inproceedings{cc6337834e8c4bbe87e3a6931e143846,

title = "Cell behaviors mediated by the scale of extracellular matrix micro-islands",

abstract = "Extracellular matrix (ECM) plays a critical role in regulating cell behaviors such as proliferation, spreading, migration and differentiation. Here we investigate ECM micro-patterns as a factor of cell-ECM interactions. Different sizes of circular ECM islands were fabricated using the microcontact printing (μCP) technique as an array of cell attachment sites spreading over the substrates. We investigate the scale of ECM islands on their effects on cell morphology and the focal adhesions (FAs) characteristics. Further, we observe morphological changes and cytoskeleton formation during the cell spreading stage of cells on different ECM patterns, revealing that the size and gap distance between the patterns influences the cell spreading as well as the dynamic cytoskeleton architecture significantly.",

author = "Shuhuan Hu and Lam, \{Raymond H.W.\}",

year = "2017",

month = mar,

day = "20",

doi = "10.1109/NANOMED.2016.7883489",

language = "English",

isbn = "9781509046751",

publisher = "IEEE Computer Society",

pages = "65--68",

booktitle = "IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED",

address = "United States",

note = "10th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2016 ; Conference date: 30-10-2016 Through 02-11-2016",

}

Hu, S & Lam, RHW 2017, Cell behaviors mediated by the scale of extracellular matrix micro-islands. in IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED., 7883489, IEEE Computer Society, pp. 65-68, 10th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2016, Macau, China, 30/10/16. https://doi.org/10.1109/NANOMED.2016.7883489

Cell behaviors mediated by the scale of extracellular matrix micro-islands. / Hu, Shuhuan ; Lam, Raymond H.W.
IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. IEEE Computer Society, 2017. p. 65-68 7883489.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Cell behaviors mediated by the scale of extracellular matrix micro-islands

AU - Hu, Shuhuan

AU - Lam, Raymond H.W.

PY - 2017/3/20

Y1 - 2017/3/20

N2 - Extracellular matrix (ECM) plays a critical role in regulating cell behaviors such as proliferation, spreading, migration and differentiation. Here we investigate ECM micro-patterns as a factor of cell-ECM interactions. Different sizes of circular ECM islands were fabricated using the microcontact printing (μCP) technique as an array of cell attachment sites spreading over the substrates. We investigate the scale of ECM islands on their effects on cell morphology and the focal adhesions (FAs) characteristics. Further, we observe morphological changes and cytoskeleton formation during the cell spreading stage of cells on different ECM patterns, revealing that the size and gap distance between the patterns influences the cell spreading as well as the dynamic cytoskeleton architecture significantly.

AB - Extracellular matrix (ECM) plays a critical role in regulating cell behaviors such as proliferation, spreading, migration and differentiation. Here we investigate ECM micro-patterns as a factor of cell-ECM interactions. Different sizes of circular ECM islands were fabricated using the microcontact printing (μCP) technique as an array of cell attachment sites spreading over the substrates. We investigate the scale of ECM islands on their effects on cell morphology and the focal adhesions (FAs) characteristics. Further, we observe morphological changes and cytoskeleton formation during the cell spreading stage of cells on different ECM patterns, revealing that the size and gap distance between the patterns influences the cell spreading as well as the dynamic cytoskeleton architecture significantly.

UR - https://www.scopus.com/pages/publications/85018598001

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85018598001&origin=recordpage

U2 - 10.1109/NANOMED.2016.7883489

DO - 10.1109/NANOMED.2016.7883489

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781509046751

SP - 65

EP - 68

BT - IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED

PB - IEEE Computer Society

T2 - 10th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2016

Y2 - 30 October 2016 through 2 November 2016

ER -

Cell behaviors mediated by the scale of extracellular matrix micro-islands

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