Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection

Guoxin Jiang; Andrei S. Susha; Andrey A. Lutich; Fernando D. Stefani; Jochen Feldmann; Andrey L. Rogach

doi:10.1021/nn901324y

Author(s)

Guoxin Jiang
Andrei S. Susha
Andrey A. Lutich
Fernando D. Stefani
Jochen Feldmann

Detail(s)

Original language	English
Pages (from-to)	4127-4131
Journal / Publication	ACS Nano
Volume	3
Issue number	12
Publication status	Published - 22 Dec 2009

Link(s)

DOI	DOI https://doi.org/10.1021/nn901324y Final Published version
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Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-73849099062&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(89b7bd6a-8cf9-4ca8-a3f8-8920e4436559).html

Abstract

Electrostatic complexes of a water-soluble fluorescent conjugated polymer, poly[9,9-bis(3′-((N,N-dimethyl)-N-ethylammonium)propyl)-2, 7-fluorene-alt-1,4-phenylene]dibromide (PDFD), and water-soluble CdTe quantum dots (QDs) are designed to provide a cascaded FRET for DNA hybridization detection. PDFD has two functions in the detection scheme: as a light-harvesting antenna, it enhances the emission of QDs by the first level FRET and inverts the sign of the surface charge of QDs, thus providing a positively charged surface to allow negatively charged dye-labeled DNA to interact with the resulting complex. This interaction causes the second level FRET to infrared-emitting dye labeled on the probe DNA, providing a reliable signal-on sensing platform discriminating between complementary and non-complementary DNA. A detailed spectroscopic study offers a clear description of photophysical processes in the designed polymer/QD/DNA complex, providing ample potential for further sensitivity and selectivity improvements. © 2009 American Chemical Society.

Research Area(s)

Conjugated polymers, DNA hybridization, FRET, Homogeneous assay, Semiconductor nanocrystals

Citation Format(s)

[ RIS ] [ BibTeX ]

Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection. / Jiang, Guoxin; Susha, Andrei S.; Lutich, Andrey A. et al.
In: ACS Nano, Vol. 3, No. 12, 22.12.2009, p. 4127-4131.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Jiang, G, Susha, AS, Lutich, AA, Stefani, FD, Feldmann, J & Rogach, AL 2009, 'Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection', ACS Nano, vol. 3, no. 12, pp. 4127-4131. https://doi.org/10.1021/nn901324y

Jiang, G., Susha, A. S., Lutich, A. A., Stefani, F. D., Feldmann, J., & Rogach, A. L. (2009). Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection. ACS Nano, 3(12), 4127-4131. https://doi.org/10.1021/nn901324y

Jiang G, Susha AS, Lutich AA, Stefani FD, Feldmann J, Rogach AL. Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection. ACS Nano. 2009 Dec 22;3(12):4127-4131. doi: 10.1021/nn901324y

Jiang, Guoxin ; Susha, Andrei S. ; Lutich, Andrey A. et al. / Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection. In: ACS Nano. 2009 ; Vol. 3, No. 12. pp. 4127-4131.