Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Guoxin Jiang
  • Andrei S. Susha
  • Andrey A. Lutich
  • Fernando D. Stefani
  • Jochen Feldmann

Detail(s)

Original languageEnglish
Pages (from-to)4127-4131
Journal / PublicationACS Nano
Volume3
Issue number12
Publication statusPublished - 22 Dec 2009

Abstract

Electrostatic complexes of a water-soluble fluorescent conjugated polymer, poly[9,9-bis(3′-((N,N-dimethyl)-N-ethylammonium)propyl)-2, 7-fluorene-alt-1,4-phenylene]dibromide (PDFD), and water-soluble CdTe quantum dots (QDs) are designed to provide a cascaded FRET for DNA hybridization detection. PDFD has two functions in the detection scheme: as a light-harvesting antenna, it enhances the emission of QDs by the first level FRET and inverts the sign of the surface charge of QDs, thus providing a positively charged surface to allow negatively charged dye-labeled DNA to interact with the resulting complex. This interaction causes the second level FRET to infrared-emitting dye labeled on the probe DNA, providing a reliable signal-on sensing platform discriminating between complementary and non-complementary DNA. A detailed spectroscopic study offers a clear description of photophysical processes in the designed polymer/QD/DNA complex, providing ample potential for further sensitivity and selectivity improvements. © 2009 American Chemical Society.

Research Area(s)

  • Conjugated polymers, DNA hybridization, FRET, Homogeneous assay, Semiconductor nanocrystals

Citation Format(s)

Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection. / Jiang, Guoxin; Susha, Andrei S.; Lutich, Andrey A.; Stefani, Fernando D.; Feldmann, Jochen; Rogach, Andrey L.

In: ACS Nano, Vol. 3, No. 12, 22.12.2009, p. 4127-4131.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review