Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection

Guoxin Jiang; Andrei S. Susha; Andrey A. Lutich; Fernando D. Stefani; Jochen Feldmann; Andrey L. Rogach

doi:10.1021/nn901324y

Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection

Guoxin Jiang, Andrei S. Susha, Andrey A. Lutich, Fernando D. Stefani, Jochen Feldmann, Andrey L. Rogach

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

181 Citations (Scopus)

Abstract

Electrostatic complexes of a water-soluble fluorescent conjugated polymer, poly[9,9-bis(3′-((N,N-dimethyl)-N-ethylammonium)propyl)-2, 7-fluorene-alt-1,4-phenylene]dibromide (PDFD), and water-soluble CdTe quantum dots (QDs) are designed to provide a cascaded FRET for DNA hybridization detection. PDFD has two functions in the detection scheme: as a light-harvesting antenna, it enhances the emission of QDs by the first level FRET and inverts the sign of the surface charge of QDs, thus providing a positively charged surface to allow negatively charged dye-labeled DNA to interact with the resulting complex. This interaction causes the second level FRET to infrared-emitting dye labeled on the probe DNA, providing a reliable signal-on sensing platform discriminating between complementary and non-complementary DNA. A detailed spectroscopic study offers a clear description of photophysical processes in the designed polymer/QD/DNA complex, providing ample potential for further sensitivity and selectivity improvements. © 2009 American Chemical Society.

Original language	English
Pages (from-to)	4127-4131
Journal	ACS Nano
Volume	3
Issue number	12
DOIs	https://doi.org/10.1021/nn901324y
Publication status	Published - 22 Dec 2009

Research Keywords

Conjugated polymers
DNA hybridization
FRET
Homogeneous assay
Semiconductor nanocrystals

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title = "Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection",

abstract = "Electrostatic complexes of a water-soluble fluorescent conjugated polymer, poly[9,9-bis(3′-((N,N-dimethyl)-N-ethylammonium)propyl)-2, 7-fluorene-alt-1,4-phenylene]dibromide (PDFD), and water-soluble CdTe quantum dots (QDs) are designed to provide a cascaded FRET for DNA hybridization detection. PDFD has two functions in the detection scheme: as a light-harvesting antenna, it enhances the emission of QDs by the first level FRET and inverts the sign of the surface charge of QDs, thus providing a positively charged surface to allow negatively charged dye-labeled DNA to interact with the resulting complex. This interaction causes the second level FRET to infrared-emitting dye labeled on the probe DNA, providing a reliable signal-on sensing platform discriminating between complementary and non-complementary DNA. A detailed spectroscopic study offers a clear description of photophysical processes in the designed polymer/QD/DNA complex, providing ample potential for further sensitivity and selectivity improvements. {\textcopyright} 2009 American Chemical Society.",

keywords = "Conjugated polymers, DNA hybridization, FRET, Homogeneous assay, Semiconductor nanocrystals",

author = "Guoxin Jiang and Susha, {Andrei S.} and Lutich, {Andrey A.} and Stefani, {Fernando D.} and Jochen Feldmann and Rogach, {Andrey L.}",

year = "2009",

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doi = "10.1021/nn901324y",

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T1 - Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection

AU - Jiang, Guoxin

AU - Susha, Andrei S.

AU - Lutich, Andrey A.

AU - Stefani, Fernando D.

AU - Feldmann, Jochen

AU - Rogach, Andrey L.

PY - 2009/12/22

Y1 - 2009/12/22

N2 - Electrostatic complexes of a water-soluble fluorescent conjugated polymer, poly[9,9-bis(3′-((N,N-dimethyl)-N-ethylammonium)propyl)-2, 7-fluorene-alt-1,4-phenylene]dibromide (PDFD), and water-soluble CdTe quantum dots (QDs) are designed to provide a cascaded FRET for DNA hybridization detection. PDFD has two functions in the detection scheme: as a light-harvesting antenna, it enhances the emission of QDs by the first level FRET and inverts the sign of the surface charge of QDs, thus providing a positively charged surface to allow negatively charged dye-labeled DNA to interact with the resulting complex. This interaction causes the second level FRET to infrared-emitting dye labeled on the probe DNA, providing a reliable signal-on sensing platform discriminating between complementary and non-complementary DNA. A detailed spectroscopic study offers a clear description of photophysical processes in the designed polymer/QD/DNA complex, providing ample potential for further sensitivity and selectivity improvements. © 2009 American Chemical Society.

AB - Electrostatic complexes of a water-soluble fluorescent conjugated polymer, poly[9,9-bis(3′-((N,N-dimethyl)-N-ethylammonium)propyl)-2, 7-fluorene-alt-1,4-phenylene]dibromide (PDFD), and water-soluble CdTe quantum dots (QDs) are designed to provide a cascaded FRET for DNA hybridization detection. PDFD has two functions in the detection scheme: as a light-harvesting antenna, it enhances the emission of QDs by the first level FRET and inverts the sign of the surface charge of QDs, thus providing a positively charged surface to allow negatively charged dye-labeled DNA to interact with the resulting complex. This interaction causes the second level FRET to infrared-emitting dye labeled on the probe DNA, providing a reliable signal-on sensing platform discriminating between complementary and non-complementary DNA. A detailed spectroscopic study offers a clear description of photophysical processes in the designed polymer/QD/DNA complex, providing ample potential for further sensitivity and selectivity improvements. © 2009 American Chemical Society.

KW - Conjugated polymers

KW - DNA hybridization

KW - FRET

KW - Homogeneous assay

KW - Semiconductor nanocrystals

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DO - 10.1021/nn901324y

M3 - RGC 21 - Publication in refereed journal

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JO - ACS Nano

JF - ACS Nano

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ER -

Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection

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