Carbon Dot Nanothermometry : Intracellular Photoluminescence Lifetime Thermal Sensing

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Sergii Kalytchuk
  • Kateřina Poláková
  • Yu Wang
  • Jens P. Froning
  • Klara Cepe
  • Radek Zbořil

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)1432-1442
Journal / PublicationACS Nano
Volume11
Issue number2
Online published26 Jan 2017
Publication statusPublished - 28 Feb 2017

Link(s)

Abstract

Nanoscale biocompatible photoluminescence (PL) thermometers that can be used to accurately and reliably monitor intracellular temperatures have many potential applications in biology and medicine. Ideally, such nanothermometers should be functional at physiological pH across a wide range of ionic strengths, probe concentrations, and local environments. Here, we show that water-soluble N,S-co-doped carbon dots (CDs) exhibit temperature-dependent photoluminescence lifetimes and can serve as highly sensitive and reliable intracellular nanothermometers. PL intensity measurements indicate that these CDs have many advantages over alternative semiconductor- and CD-based nanoscale temperature sensors. Importantly, their PL lifetimes remain constant over wide ranges of pH values (5-12), CD concentrations (1.5 × 10-5 to 0.5 mg/mL), and environmental ionic strengths (up to 0.7 mol·L-1 NaCl). Moreover, they are biocompatible and nontoxic, as demonstrated by cell viability and flow cytometry analyses using NIH/3T3 and HeLa cell lines. N,S-CD thermal sensors also exhibit good water dispersibility, superior photo- and thermostability, extraordinary environment and concentration independence, high storage stability, and reusability-their PL decay curves at temperatures between 15 and 45 °C remained unchanged over seven sequential experiments. In vitro PL lifetime-based temperature sensing performed with human cervical cancer HeLa cells demonstrated the great potential of these nanosensors in biomedicine. Overall, N,S-doped CDs exhibit excitation-independent emission with strongly temperature-dependent monoexponential decay, making them suitable for both in vitro and in vivo luminescence lifetime thermometry.

Research Area(s)

  • carbon dots, colloidal nanomaterials, HeLa cancer cells, intracellular temperature, luminescence lifetime thermal sensing, nanothermometer, photoluminescence

Citation Format(s)

Carbon Dot Nanothermometry : Intracellular Photoluminescence Lifetime Thermal Sensing. / Kalytchuk, Sergii; Poláková, Kateřina; Wang, Yu et al.

In: ACS Nano, Vol. 11, No. 2, 28.02.2017, p. 1432-1442.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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