Capillary electrophoretic method for determination of matrine in caco-2 cell medium

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)2861-2870
Journal / PublicationJournal of Liquid Chromatography and Related Technologies
Volume27
Issue number18
Publication statusPublished - 2004
Externally publishedYes

Abstract

A capillary electrophoretic (CE) method has been developed for the analysis of matrine in a biological medium. The optimized run buffer solution contained 200 mM tris(hydroxymethyl)aminomethane, 40 mM sodium dihydrogen phosphate, and 20% 2-propanol, adjusted to pH 5.8 using phosphoric acid. A voltage of 25 kV was applied across a capillary (50 μm × 40 cm, distance to detector: 10.2cm) for CE separation. Each analysis was completed in less than 5 min. Linearity of matrine calibration in the concentration range of 3.2-185 μg/mL (at the detection wavelength of 214 nm) was excellent (R2 = 0.9994). The run-to-run repeatability (n = 3), as expressed by the relative standard deviation (RSD), was found to be better than 4.0%. The limit of detection was estimated to be 3.2 μ/mL. This CE method was employed to determine matrine in Caco-2 cell media after transport of matrine through the cells.

Research Area(s)

  • Caco-2 cell, Capillary electrophoresis, Matrine

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