Canine intestinal spirochetes consist of Serpulina pilosicoli and a newly identified group provisionally designated "Serpulina canis" sp. nov.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • G. E. DUHAMEL
  • D. J. TROTT
  • N. MUNIAPPA
  • M. R. MATHIESEN
  • K. TARASIUK
  • J. I. LEE

Detail(s)

Original languageEnglish
Pages (from-to)2264-2270
Journal / PublicationJournal of Clinical Microbiology
Volume36
Issue number8
Publication statusPublished - Aug 1998
Externally publishedYes

Abstract

The spirochetes inhabiting the large intestines of humans and animals consist of a diverse group of related organisms. Intestinal spirochetosis caused by Serpulina pilosicoli is a newly recognized enteric disease of human beings and animals with potential public health significance. The purpose of this study was to determine the species identity of canine intestinal spirochetes by comparing 30 isolates obtained from dogs in Australia (n = 25) and the United States (n = 5) with reference strains representing Serpulina species and Brachyspira aalborgi, by phenotypic and genetically based typing methods. All of the canine isolates were indole negative and produced a weak β-hemolysis when cultured anaerobically on agar medium containing blood. Four isolates were identified as S. pilosicoli by 16S rRNA-specific PCR assays, rRNA gene restriction fragment length polymorphism or ribotyping, and multilocus enzyme electrophoresis. The remaining 26 isolates formed a cluster related to porcine Serpulina innocens as determined by multilocus enzyme electrophoresis but had a unique ribotype pattern. The data suggested the existence of a novel Serpulina species, provisionally designated “Serpulina canis,” colonizing the intestines of dogs.

Citation Format(s)

Canine intestinal spirochetes consist of Serpulina pilosicoli and a newly identified group provisionally designated "Serpulina canis" sp. nov. / DUHAMEL, G. E.; TROTT, D. J.; MUNIAPPA, N.; MATHIESEN, M. R.; TARASIUK, K.; LEE, J. I.; HAMPSON, D. J.

In: Journal of Clinical Microbiology, Vol. 36, No. 8, 08.1998, p. 2264-2270.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review