Canaliculi in the tessellated skeleton of cartilaginous fishes

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

26 Scopus Citations
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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)263-267
Journal / PublicationJournal of Applied Ichthyology
Volume26
Issue number2
Online published13 Apr 2010
Publication statusPublished - Apr 2010
Externally publishedYes

Abstract

The endoskeletal elements of sharks and rays are comprised of an uncalcified, hyaline cartilage-like core overlain by a thin fibro-ceramic layer of mineralized hexagonal tiles (tesserae) adjoined by intertesseral fibers. The basic spatial relationships of the constituent tissues (unmineralized cartilage, mineralized cartilage, fibrous tissue) are well-known - endoskeletal tessellation is a long-recognized synapomorphy of elasmobranch fishes - but a high-resolution and three-dimensional (3D) understanding of their interactions has been hampered by difficulties in sample preparation and lack of technologies adequate for visualizing microstructure and microassociations. We used cryo-electron microscopy and synchrotron radiation tomography to investigate tessellated skeleton ultrastructure but without damage to the delicate relationships between constituent tissues or to the tesserae themselves. The combination of these techniques allowed visualization of never before appreciated internal structures, namely passages connecting the lacunar spaces within tesserae. These intratesseral 'canaliculi' link consecutive lacunar spaces into long lacunar strings, radiating outward from the center of tesserae. The continuity of extracellular matrix throughout the canalicular network may explain how chondrocytes in tesserae remain vital despite encasement in mineral. Extracellular fluid exchange may also permit transmission of nutrients, and mechanical and mineralization signals among chondrocytes, in a manner similar to the canalicular network in bone. These co-adapted mechanisms for the facilitated exchange of extracellular material suggest a level of parallelism in early chondrocyte and osteocyte evolution. © 2010 Blackwell Verlag, Berlin.

Citation Format(s)

Canaliculi in the tessellated skeleton of cartilaginous fishes. / Dean, M. N.; Socha, J. J.; Hall, B. K. et al.
In: Journal of Applied Ichthyology, Vol. 26, No. 2, 04.2010, p. 263-267.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review