A cloud-based e-learning tool, LectureTools, was piloted at City University of Hong Kong in the year 2012-13. LectureTools is a cloud-based teaching and learning platform that combines the features of interactive presentation, personal response system (PRS), student inquiry and online note-taking into one system. Students are able to access to the lecture materials and response to in-class questions via LectureTools using any web-enabled devices such as notebook computers, tablets, or even smartphones, providing them a flexible and comfortable learning environment. LectureTools can summarize students’ in-class responses and displays them in real-time, delivering timely formative feedback to instructors. A comprehensive study is conducted to investigate the effectiveness of LectureTools on enhancing student learning motivation. A group of graduate students (n=158) was participated in this work and subjected to a psychometric instrument, the Motivated Strategies for Learning Questionnaire (MSLQ), which is based on the cognitive view of motivation. MSLQ results showed that when students were engaged with LectureTools in-class, the reported values of all six learning motivation scales of MSLQ increased, namely intrinsic goal orientation, extrinsic goal orientation, task value, control beliefs, self-efficacy for learning & performance, and test anxiety. In particular, the reported intrinsic goal orientation value was statistically significant from those who did not use the tool. Instructors’ qualitative feedback indicated that the e-learning tool was useful to engage passive students and the interactive online question with real-time result display function was the best part of the system, while the in-class student inquiry function was difficult to use in practice.