Bright Near-Infrared π-Conjugated Oligomer Nanoparticles for Deep-Brain Three-Photon Microscopy Excited at the 1700 nm Window in Vivo

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

3 Scopus Citations
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Author(s)

  • Shengliang Li
  • Xiangquan Deng
  • Hui Cheng
  • Jie Yu
  • Ying Liu
  • Yi Yuan
  • Ke Wang

Detail(s)

Original languageEnglish
Pages (from-to)12480-12487
Journal / PublicationACS Nano
Volume16
Issue number8
Online published15 Aug 2022
Publication statusPublished - 23 Aug 2022

Abstract

The development of three-photon fluorophores with 1700 nm excitation is pressingly desirable for in vivo imaging of tissue resided deep inside the brain. Herein, we report a designed and synthesized fluorescent molecule (OFET) for in vivo mouse brain imaging with three-photon microscopy at a record imaging depth. The OFET molecule has a relatively high fluorescence brightness and has a near-infrared (NIR) maximum emission at 820 nm after integrating as water-dispersible nanoparticles (OEFT NPs). Under 1720 nm excitation, OFET NPs show a large three-photon action cross-section of 1.06 × 10-82 cms2/photon2, which is more than twice that of the commonly used sulforhodamine 101 (SR101) dye. Benefiting from the high tissue penetration depths for both the long excitation in the second NIR window of 1720 nm and the emission wavelength in the first NIR window of 820 nm, a high brightness, and a large action cross-section of three-photon, OFET NPs have good deep-brain imaging performance. Brain vasculatures of a mouse located at a depth of 1696 μm can be clearly resolved in vivo. With no observable cytotoxicity even in a high concentration, the present OFET NPs suggest that fluorescent π-conjugated oligomers are of great potential in high-resolution 3PM imaging of in vivo deep-tissue.

Research Area(s)

  • conjugated oligomer, near-infrared, three-photon microscopy, brain imaging, 1700 nm window, POLYMER DOTS, FLUORESCENCE

Citation Format(s)

Bright Near-Infrared π-Conjugated Oligomer Nanoparticles for Deep-Brain Three-Photon Microscopy Excited at the 1700 nm Window in Vivo. / Li, Shengliang; Deng, Xiangquan; Cheng, Hui et al.

In: ACS Nano, Vol. 16, No. 8, 23.08.2022, p. 12480-12487.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review