TY - JOUR
T1 - Bright Near-Infrared π-Conjugated Oligomer Nanoparticles for Deep-Brain Three-Photon Microscopy Excited at the 1700 nm Window in Vivo
AU - Li, Shengliang
AU - Deng, Xiangquan
AU - Cheng, Hui
AU - Li, Xiaozhen
AU - Wan, Yingpeng
AU - Cao, Chen
AU - Yu, Jie
AU - Liu, Ying
AU - Yuan, Yi
AU - Wang, Ke
AU - Lee, Chun-Sing
PY - 2022/8/23
Y1 - 2022/8/23
N2 - The development of three-photon fluorophores with 1700 nm excitation is pressingly desirable for in vivo imaging of tissue resided deep inside the brain. Herein, we report a designed and synthesized fluorescent molecule (OFET) for in vivo mouse brain imaging with three-photon microscopy at a record imaging depth. The OFET molecule has a relatively high fluorescence brightness and has a near-infrared (NIR) maximum emission at 820 nm after integrating as water-dispersible nanoparticles (OEFT NPs). Under 1720 nm excitation, OFET NPs show a large three-photon action cross-section of 1.06 × 10-82 cm6 s2/photon2, which is more than twice that of the commonly used sulforhodamine 101 (SR101) dye. Benefiting from the high tissue penetration depths for both the long excitation in the second NIR window of 1720 nm and the emission wavelength in the first NIR window of 820 nm, a high brightness, and a large action cross-section of three-photon, OFET NPs have good deep-brain imaging performance. Brain vasculatures of a mouse located at a depth of 1696 μm can be clearly resolved in vivo. With no observable cytotoxicity even in a high concentration, the present OFET NPs suggest that fluorescent π-conjugated oligomers are of great potential in high-resolution 3PM imaging of in vivo deep-tissue.
AB - The development of three-photon fluorophores with 1700 nm excitation is pressingly desirable for in vivo imaging of tissue resided deep inside the brain. Herein, we report a designed and synthesized fluorescent molecule (OFET) for in vivo mouse brain imaging with three-photon microscopy at a record imaging depth. The OFET molecule has a relatively high fluorescence brightness and has a near-infrared (NIR) maximum emission at 820 nm after integrating as water-dispersible nanoparticles (OEFT NPs). Under 1720 nm excitation, OFET NPs show a large three-photon action cross-section of 1.06 × 10-82 cm6 s2/photon2, which is more than twice that of the commonly used sulforhodamine 101 (SR101) dye. Benefiting from the high tissue penetration depths for both the long excitation in the second NIR window of 1720 nm and the emission wavelength in the first NIR window of 820 nm, a high brightness, and a large action cross-section of three-photon, OFET NPs have good deep-brain imaging performance. Brain vasculatures of a mouse located at a depth of 1696 μm can be clearly resolved in vivo. With no observable cytotoxicity even in a high concentration, the present OFET NPs suggest that fluorescent π-conjugated oligomers are of great potential in high-resolution 3PM imaging of in vivo deep-tissue.
KW - conjugated oligomer
KW - near-infrared
KW - three-photon microscopy
KW - brain imaging
KW - 1700 nm window
KW - POLYMER DOTS
KW - FLUORESCENCE
UR - http://www.scopus.com/inward/record.url?scp=85136618807&partnerID=8YFLogxK
UR - http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000844862600001
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85136618807&origin=recordpage
U2 - 10.1021/acsnano.2c03813
DO - 10.1021/acsnano.2c03813
M3 - RGC 21 - Publication in refereed journal
SN - 1936-0851
VL - 16
SP - 12480
EP - 12487
JO - ACS Nano
JF - ACS Nano
IS - 8
ER -