Bone integration capability of a series of strontium-containing hydroxyapatite coatings formed by micro-arc oxidation

Strontium-containing hydroxyapatites (Sr-HA) combine the desirable bone regenerative properties of hydroxyapatites (HA) with anabolic and anti-catabolic effects of strontium cations. In the present work, a series of Sr_yHA [Sr_yCa_(10-y)(PO₄)₆(OH)₂; y = 0, 0.5, 1, 2] coatings on titanium are produced by micro-arc oxidation (MAO), and the effects of the in vivo osseointegration ability of the coatings are investigated by using a rabbit model. All samples are subjected to biomechanical, surface elemental, micro-CT and histological analysis after 4 and 12 weeks of healing. The obtained results show that the MAO-formed coatings exhibit a microporous network structure composed of Sr_yHA/Sr _yHA-Sr_xCa_(1-x)TiO₃/Sr _xCa_(1-x)TiO₃-TiO₂ multilayers, in which the outer Sr_yHA and intermediate Sr_yHA-Sr _xCa_(1-x)TiO₃ layers have a nanocrystalline structure. All Sr-HA coated implants induce marked improvements in the behavior of bone formation, quantity and quality of bone tissue around the implants than the control HA implant and in particular, the 20%Sr-HA coating promotes early bone formation as identified by polyfluorochrome sequential labeling. The bone-to-implant contact is increased by 46% (p <0.05) and the pull-out strength is increased by 103% over the HA group (p <0.01). Extensive areas of mineralized tissue densely deposit on the 20%Sr-HA coating after biomechanical testing, and the greatest improvement of bone microarchitecture are observed around the 20%Sr-HA implant. The identified biological parameters successfully demonstrate the osteoconductivity of 20%Sr-HA surfaces, which results not only in an acceleration but also an improvement of bone-implant integration. The study demonstrates the immense potential of 20%Sr-HA coatings in dental and orthopedic applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2465-2480, 2013. Copyright © 2012 Wiley Periodicals, Inc.