Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy

Rafay Ahmed; Alan Wing Lun Law; Tsz Wing Cheung; Condon Lau

doi:10.1117/12.2283272

Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy

Rafay Ahmed
, Alan Wing Lun Law
, Tsz Wing Cheung
, Condon Lau^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Subcritical calvarial defects are important to study bone regeneration during healing. In this study 1mm calvarial defects were created using trephine in the parietal bones of Sprague-Dawley rats (n=7) that served as in vivo defects. Subjects were sacrificed after 7 days and the additional defects were created on the harvested skull with the same method to serve as control defects. Raman spectroscopy is established to investigate mineral/matrix ratio, carbonate/phosphate ratio & crystallinity of three different surfaces; in vivo defects, control defects and normal surface. Results show 21% and 23% decrease in mineral/matrix after 7 days of healing from surface to in vivo and control to in vivo defects, respectively. Carbonate to phosphate ratio was found to be increased by 39% while crystallinity decreased by 26% in both surface to in vivo and control to in vivo defects. This model allows to study the regenerated bone without mechanically perturbing healing surface.

Original language	English
Title of host publication	Medical Laser Applications and Laser-Tissue Interactions VIII
Editors	Lothar D. Lilge, Ronald Sroka
Publisher	SPIE
ISBN (Electronic)	9781510612938
ISBN (Print)	9781510612921
DOIs	https://doi.org/10.1117/12.2283272
Publication status	Published - Jun 2017
Event	European Conferences on Biomedical Optics (ECBO) - ICM—International Congress Centre, Munich, Germany Duration: 25 Jun 2017 → 29 Jun 2017 https://www.gophotonics.com/events/details/194-european-conference-on-biomedical-optics-ecbo-

Publication series

Name	Proceedings of SPIE
Volume	10417
ISSN (Print)	1605-7422

Conference

Conference	European Conferences on Biomedical Optics (ECBO)
Place	Germany
City	Munich
Period	25/06/17 → 29/06/17
Internet address	https://www.gophotonics.com/events/details/194-european-conference-on-biomedical-optics-ecbo-

Research Keywords

Bone fracture healing
Calvarial defects
Raman spectroscopy

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10.1117/12.2283272

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@inproceedings{a4d386989f9a4c2e9722d66b8d3236ee,

title = "Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy",

abstract = "Subcritical calvarial defects are important to study bone regeneration during healing. In this study 1mm calvarial defects were created using trephine in the parietal bones of Sprague-Dawley rats (n=7) that served as in vivo defects. Subjects were sacrificed after 7 days and the additional defects were created on the harvested skull with the same method to serve as control defects. Raman spectroscopy is established to investigate mineral/matrix ratio, carbonate/phosphate ratio \& crystallinity of three different surfaces; in vivo defects, control defects and normal surface. Results show 21\% and 23\% decrease in mineral/matrix after 7 days of healing from surface to in vivo and control to in vivo defects, respectively. Carbonate to phosphate ratio was found to be increased by 39\% while crystallinity decreased by 26\% in both surface to in vivo and control to in vivo defects. This model allows to study the regenerated bone without mechanically perturbing healing surface.",

keywords = "Bone fracture healing, Calvarial defects, Raman spectroscopy",

author = "Rafay Ahmed and Law, \{Alan Wing Lun\} and Cheung, \{Tsz Wing\} and Condon Lau",

year = "2017",

month = jun,

doi = "10.1117/12.2283272",

language = "English",

isbn = "9781510612921",

series = "Proceedings of SPIE",

publisher = "SPIE",

editor = "Lilge, \{Lothar D.\} and Ronald Sroka",

booktitle = "Medical Laser Applications and Laser-Tissue Interactions VIII",

address = "United States",

note = "European Conferences on Biomedical Optics (ECBO) ; Conference date: 25-06-2017 Through 29-06-2017",

url = "https://www.gophotonics.com/events/details/194-european-conference-on-biomedical-optics-ecbo-",

}

Ahmed, R, Law, AWL, Cheung, TW & Lau, C 2017, Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy. in LD Lilge & R Sroka (eds), Medical Laser Applications and Laser-Tissue Interactions VIII., 104170O, Proceedings of SPIE, vol. 10417, SPIE, European Conferences on Biomedical Optics (ECBO), Munich, Germany, 25/06/17. https://doi.org/10.1117/12.2283272

Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy. / Ahmed, Rafay; Law, Alan Wing Lun; Cheung, Tsz Wing et al.
Medical Laser Applications and Laser-Tissue Interactions VIII. ed. / Lothar D. Lilge; Ronald Sroka. SPIE, 2017. 104170O (Proceedings of SPIE; Vol. 10417).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy

AU - Ahmed, Rafay

AU - Law, Alan Wing Lun

AU - Cheung, Tsz Wing

AU - Lau, Condon

PY - 2017/6

Y1 - 2017/6

N2 - Subcritical calvarial defects are important to study bone regeneration during healing. In this study 1mm calvarial defects were created using trephine in the parietal bones of Sprague-Dawley rats (n=7) that served as in vivo defects. Subjects were sacrificed after 7 days and the additional defects were created on the harvested skull with the same method to serve as control defects. Raman spectroscopy is established to investigate mineral/matrix ratio, carbonate/phosphate ratio & crystallinity of three different surfaces; in vivo defects, control defects and normal surface. Results show 21% and 23% decrease in mineral/matrix after 7 days of healing from surface to in vivo and control to in vivo defects, respectively. Carbonate to phosphate ratio was found to be increased by 39% while crystallinity decreased by 26% in both surface to in vivo and control to in vivo defects. This model allows to study the regenerated bone without mechanically perturbing healing surface.

AB - Subcritical calvarial defects are important to study bone regeneration during healing. In this study 1mm calvarial defects were created using trephine in the parietal bones of Sprague-Dawley rats (n=7) that served as in vivo defects. Subjects were sacrificed after 7 days and the additional defects were created on the harvested skull with the same method to serve as control defects. Raman spectroscopy is established to investigate mineral/matrix ratio, carbonate/phosphate ratio & crystallinity of three different surfaces; in vivo defects, control defects and normal surface. Results show 21% and 23% decrease in mineral/matrix after 7 days of healing from surface to in vivo and control to in vivo defects, respectively. Carbonate to phosphate ratio was found to be increased by 39% while crystallinity decreased by 26% in both surface to in vivo and control to in vivo defects. This model allows to study the regenerated bone without mechanically perturbing healing surface.

KW - Bone fracture healing

KW - Calvarial defects

KW - Raman spectroscopy

UR - https://www.scopus.com/pages/publications/85033384018

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85033384018&origin=recordpage

U2 - 10.1117/12.2283272

DO - 10.1117/12.2283272

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781510612921

T3 - Proceedings of SPIE

BT - Medical Laser Applications and Laser-Tissue Interactions VIII

A2 - Lilge, Lothar D.

A2 - Sroka, Ronald

PB - SPIE

T2 - European Conferences on Biomedical Optics (ECBO)

Y2 - 25 June 2017 through 29 June 2017

ER -

Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy

Abstract

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Diagnosing Healing Calvarial Defects through Spectroscopic Methods

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Bone compositional study during healing of subcritical calvarial defects in rats by Raman spectroscopy

Abstract

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Conference

Research Keywords

Access Output

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Student theses

Diagnosing Healing Calvarial Defects through Spectroscopic Methods

Cite this