Blockage of hydroxyl group partially abolishes the cholesterol-lowering activity of β-sitosterol

Xiaobo Wang; Weihuan Huang; Lin Lei; Yuwei Liu; Ka Ying Ma; Yuk Man Li; Lijun Wang; Yu Huang; Zhen-Yu Chen

doi:10.1016/j.jff.2014.11.019

Blockage of hydroxyl group partially abolishes the cholesterol-lowering activity of β-sitosterol

Xiaobo Wang, Weihuan Huang, Lin Lei, Yuwei Liu, Ka Ying Ma, Yuk Man Li, Lijun Wang, Yu Huang, Zhen-Yu Chen^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

15 Citations (Scopus)

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Abstract

β-Sitosterol (SI) is hypocholesterolemic. The present study investigated whether the blockage of its hydroxyl group would abolish its cholesterol-lowering activity. The blockage was made by methylating and ethylating the hydroxyl group on C3 position, leading to formation of β-sitosteryl 3β-methoxy (SM) and β-sitosteryl 3β-ethoxy (SE) derivatives. Male hamsters were divided into five groups ( n = 8 each) and fed the non-cholesterol diet (NCD), high cholesterol diet containing 5 mmol of cholesterol (HCD), or one of the three high cholesterol experimental diets with addition of 5 mmol of SI (HCD + SI), 5 mmol of SM (HCD + SM) and 5 mmol of SE (HCD + SE), respectively, for 8 weeks. Results showed that SI could significantly reduce plasma total cholesterol (TC) by 17% ( P < 0.05), while SM and SE had no effect on plasma TC. It was concluded that the hydroxyl group was essential for SI to render its cholesterol-lowering activity and its blockage abolished this ability.

Original language	English
Pages (from-to)	199-207
Journal	Journal of Functional Foods
Volume	12
DOIs	https://doi.org/10.1016/j.jff.2014.11.019
Publication status	Published - 1 Jan 2015
Externally published	Yes

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Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Research Keywords

Cholesterol
β-Sitosterol
β-Sitosteryl 3β-ethoxy
β-Sitosteryl 3β-methoxy

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This full text is made available under CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/

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title = "Blockage of hydroxyl group partially abolishes the cholesterol-lowering activity of β-sitosterol",

abstract = "β-Sitosterol (SI) is hypocholesterolemic. The present study investigated whether the blockage of its hydroxyl group would abolish its cholesterol-lowering activity. The blockage was made by methylating and ethylating the hydroxyl group on C3 position, leading to formation of β-sitosteryl 3β-methoxy (SM) and β-sitosteryl 3β-ethoxy (SE) derivatives. Male hamsters were divided into five groups ( n = 8 each) and fed the non-cholesterol diet (NCD), high cholesterol diet containing 5 mmol of cholesterol (HCD), or one of the three high cholesterol experimental diets with addition of 5 mmol of SI (HCD + SI), 5 mmol of SM (HCD + SM) and 5 mmol of SE (HCD + SE), respectively, for 8 weeks. Results showed that SI could significantly reduce plasma total cholesterol (TC) by 17% ( P < 0.05), while SM and SE had no effect on plasma TC. It was concluded that the hydroxyl group was essential for SI to render its cholesterol-lowering activity and its blockage abolished this ability.",

keywords = "Cholesterol, β-Sitosterol, β-Sitosteryl 3β-ethoxy, β-Sitosteryl 3β-methoxy",

author = "Xiaobo Wang and Weihuan Huang and Lin Lei and Yuwei Liu and Ma, {Ka Ying} and Li, {Yuk Man} and Lijun Wang and Yu Huang and Zhen-Yu Chen",

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TY - JOUR

T1 - Blockage of hydroxyl group partially abolishes the cholesterol-lowering activity of β-sitosterol

AU - Wang, Xiaobo

AU - Huang, Weihuan

AU - Lei, Lin

AU - Liu, Yuwei

AU - Ma, Ka Ying

AU - Li, Yuk Man

AU - Wang, Lijun

AU - Huang, Yu

AU - Chen, Zhen-Yu

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2015/1/1

Y1 - 2015/1/1

N2 - β-Sitosterol (SI) is hypocholesterolemic. The present study investigated whether the blockage of its hydroxyl group would abolish its cholesterol-lowering activity. The blockage was made by methylating and ethylating the hydroxyl group on C3 position, leading to formation of β-sitosteryl 3β-methoxy (SM) and β-sitosteryl 3β-ethoxy (SE) derivatives. Male hamsters were divided into five groups ( n = 8 each) and fed the non-cholesterol diet (NCD), high cholesterol diet containing 5 mmol of cholesterol (HCD), or one of the three high cholesterol experimental diets with addition of 5 mmol of SI (HCD + SI), 5 mmol of SM (HCD + SM) and 5 mmol of SE (HCD + SE), respectively, for 8 weeks. Results showed that SI could significantly reduce plasma total cholesterol (TC) by 17% ( P < 0.05), while SM and SE had no effect on plasma TC. It was concluded that the hydroxyl group was essential for SI to render its cholesterol-lowering activity and its blockage abolished this ability.

AB - β-Sitosterol (SI) is hypocholesterolemic. The present study investigated whether the blockage of its hydroxyl group would abolish its cholesterol-lowering activity. The blockage was made by methylating and ethylating the hydroxyl group on C3 position, leading to formation of β-sitosteryl 3β-methoxy (SM) and β-sitosteryl 3β-ethoxy (SE) derivatives. Male hamsters were divided into five groups ( n = 8 each) and fed the non-cholesterol diet (NCD), high cholesterol diet containing 5 mmol of cholesterol (HCD), or one of the three high cholesterol experimental diets with addition of 5 mmol of SI (HCD + SI), 5 mmol of SM (HCD + SM) and 5 mmol of SE (HCD + SE), respectively, for 8 weeks. Results showed that SI could significantly reduce plasma total cholesterol (TC) by 17% ( P < 0.05), while SM and SE had no effect on plasma TC. It was concluded that the hydroxyl group was essential for SI to render its cholesterol-lowering activity and its blockage abolished this ability.

KW - Cholesterol

KW - β-Sitosterol

KW - β-Sitosteryl 3β-ethoxy

KW - β-Sitosteryl 3β-methoxy

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DO - 10.1016/j.jff.2014.11.019

M3 - RGC 21 - Publication in refereed journal

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SP - 199

EP - 207

JO - Journal of Functional Foods

JF - Journal of Functional Foods

ER -

Blockage of hydroxyl group partially abolishes the cholesterol-lowering activity of β-sitosterol

Abstract

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Research Keywords

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