TY - JOUR
T1 - Biosynthesis of LL-Z1272β
T2 - Discovery of a New Member of NRPS-like Enzymes for Aryl-Aldehyde Formation
AU - Li, Chang
AU - Matsuda, Yudai
AU - Gao, Hao
AU - Hu, Dan
AU - Yao, Xin Sheng
AU - Abe, Ikuro
PY - 2016/5/17
Y1 - 2016/5/17
N2 - LL-Z1272β (1) is a prenylated aryl-aldehyde produced by several fungi; it also serves as a key pathway intermediate for many fungal meroterpenoids. Despite its importance in the biosynthesis of natural products, the molecular basis for the biosynthesis of 1 has yet to be elucidated. Here we identified the biosynthetic gene cluster for 1 from Stachybotrys bisbyi PYH05-7, and elucidated the biosynthetic route to 1. The biosynthesis involves a polyketide synthase, a prenyltransferase, and a nonribosomal peptide synthetase (NRPS)-like enzyme, which is responsible for the generation of the aldehyde functionality. Interestingly, the NRPS-like enzyme only accepts the farnesylated substrate to catalyze the carboxylate reduction; this represents a new example of a substrate for adenylation domains.
AB - LL-Z1272β (1) is a prenylated aryl-aldehyde produced by several fungi; it also serves as a key pathway intermediate for many fungal meroterpenoids. Despite its importance in the biosynthesis of natural products, the molecular basis for the biosynthesis of 1 has yet to be elucidated. Here we identified the biosynthetic gene cluster for 1 from Stachybotrys bisbyi PYH05-7, and elucidated the biosynthetic route to 1. The biosynthesis involves a polyketide synthase, a prenyltransferase, and a nonribosomal peptide synthetase (NRPS)-like enzyme, which is responsible for the generation of the aldehyde functionality. Interestingly, the NRPS-like enzyme only accepts the farnesylated substrate to catalyze the carboxylate reduction; this represents a new example of a substrate for adenylation domains.
KW - aryl-aldehyde
KW - biosynthesis
KW - carboxylate reductase
KW - enzymes
KW - meroterpenoids
UR - http://www.scopus.com/inward/record.url?scp=84963713720&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84963713720&origin=recordpage
U2 - 10.1002/cbic.201600087
DO - 10.1002/cbic.201600087
M3 - RGC 21 - Publication in refereed journal
C2 - 26972702
AN - SCOPUS:84963713720
SN - 1439-4227
VL - 17
SP - 904
EP - 907
JO - ChemBioChem
JF - ChemBioChem
IS - 10
ER -