Bio-refinery of waste streams for green and efficient succinic acid production by engineered Yarrowia lipolytica without pH control

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Original languageEnglish
Pages (from-to)804-812
Journal / PublicationChemical Engineering Journal
Online published15 Apr 2019
Publication statusPublished - 1 Sep 2019


This paper presents an efficient, green and sustainable approach for succinic acid (SA) fermentation, in which glucose-rich hydrolysates from various waste streams were used as the substrate for SA production by Yarrowia lipolytica without pH control for the first time. First, Y. lipolytica PGC202 was found to be able to consume glucose-rich mixed food waste (MFW) hydrolysate for SA production with high efficiency. After medium optimization, a SA titer at 31.7 g/L with a yield of 0.52 g/g and productivity of 0.60 g/L/h was obtained from isFBB fermentation when using 60 g/L glucose-containing MFW hydrolysate as the carbon source supplemented with 3% tryptone. When glucose-rich hydrolysate from other waste streams was used, the highest SA yield of 0.61 g/g via yeast fermentation at low pH was achieved, which demonstrated the extensive substrate adaptability and great potential of Y. lipolytica. By feeding concentrated MFW hydrolysate, SA titer up to 71.6 g/L was achieved from fed-batch fermentation with pH decreased naturally to 2.8. Finally, with the revised recovery method, 68.3% of the total SA with purity between 89.5% and 91.0% was recovered from the acidic fermentation broth with only low input of acid for acidification, which demonstrated the great advantages of SA production at low pH over that under neutral conditions in terms of economic improvement and environmental sustainability. In general, this study overcomes one of the major economic drawbacks for future feasible large-scale fermentative SA production, and it also illustrates a promising prospective for bio-SA production.

Research Area(s)

  • Bio-refinery, Low pH, Succinic acid, Waste streams, Yarrowia lipolytica

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