Biomolecular screening with encoded porous-silicon photonic crystals
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 39-41 |
Journal / Publication | Nature Materials |
Volume | 1 |
Issue number | 1 |
Publication status | Published - Sept 2002 |
Externally published | Yes |
Link(s)
Abstract
Strategies to encode or label small particles or beads for use in high-throughput screening and bioassay applications' focus on either spatially differentiated, on-chip arrays2-4 or random distributions of encoded beads5,6. Attempts to encode large numbers of polymeric, metallic or glass beads in random arrays or in fluid suspension have used a variety of entities to provide coded elements (bits) - fluorescent molecules, molecules with specific vibrational signatures7,8, quantum dots9, or discrete metallic layers10. Here we report a method for optically encoding micrometre-sized nanostructured particles of porous silicon. We generate multilayered porous films in crystalline silicon using a periodic electrochemical etch. This results in photonic crystals with well-resolved and narrow optical reflectivity features, whose wavelengths are determined by the etching parameters11. Millions of possible codes can be prepared this way. Micrometre-sized particles are then produced by ultrasonic fracture12, mechanical grinding or by lithographic means. A simple antibody-based bioassay using fluorescently tagged proteins demonstrates the encoding strategy in biologically relevant media.
Citation Format(s)
Biomolecular screening with encoded porous-silicon photonic crystals. / Cunin, Frédérique; Schmedake, Thomas A.; Link, Jamie R. et al.
In: Nature Materials, Vol. 1, No. 1, 09.2002, p. 39-41.
In: Nature Materials, Vol. 1, No. 1, 09.2002, p. 39-41.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review