Bioluminescent avian pathogenic Escherichia coli for monitoring colibacillosis in experimentally infected chickens

Leon H. Oosterik; Huruma N. Tuntufye; Jessica Tsonos; Tom Luyten; Sam Noppen; Sandra Liekens; Rob Lavigne; Patrick Butaye; Bruno M. Goddeeris

doi:10.1016/j.tvjl.2016.07.011

Bioluminescent avian pathogenic Escherichia coli for monitoring colibacillosis in experimentally infected chickens

Leon H. Oosterik^*, Huruma N. Tuntufye, Jessica Tsonos, Tom Luyten, Sam Noppen, Sandra Liekens, Rob Lavigne, Patrick Butaye, Bruno M. Goddeeris

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

8 Citations (Scopus)

Abstract

Avian pathogenic Escherichia coli (APEC) are responsible for significant economic losses in the poultry industry. In this study, a model for investigating the pathogenesis of APEC infections was established. APEC strain CH2 (O78) was marked with the luciferase operon (luxCDABE) using a Tn7 transposon and tissues of experimentally infected chickens were analysed for a correlation between the bioluminescent signal and the number of bacteria. Transposition of the lux operon into the chromosome of the APEC isolate did not affect sensitivity to lytic bacteriophages and there was no effect on virulence in an intratracheal infection model in 1-day-old chicks, although results with a subcutaneous infection model were inconclusive. A correlation between the number of bacteria and the luminescent signal was found in liquid medium, as well as in homogenised heart, liver, spleen and lung of 4-week-old experimentally infected chickens. This study showed that lux could be used for identification of the infecting strain after experimental infection with APEC in poultry. © 2016 Elsevier Ltd

Original language	English
Pages (from-to)	87-92
Journal	The Veterinary Journal
Volume	216
DOIs	https://doi.org/10.1016/j.tvjl.2016.07.011
Publication status	Published - 1 Oct 2016
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

We thank Marcel Samain for the technical assistance provided during the study and are grateful to Dr. Pierre Germon for the provision of plasmid pBEN276 and thankful to Prof. Dr. Nancy Craig for the provision of plasmid pGRG25. This study was funded by the Belgian Federal Public Service of Health, Food Chain Safety and Environment (contract RT 09/8 APECON) and by CODA-CERVA (contract RP-APEC3).

Research Keywords

Avian pathogenic Escherichia coli
Bacterial luciferase
Bioluminescence
Domestic fowl

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title = "Bioluminescent avian pathogenic Escherichia coli for monitoring colibacillosis in experimentally infected chickens",

abstract = "Avian pathogenic Escherichia coli (APEC) are responsible for significant economic losses in the poultry industry. In this study, a model for investigating the pathogenesis of APEC infections was established. APEC strain CH2 (O78) was marked with the luciferase operon (luxCDABE) using a Tn7 transposon and tissues of experimentally infected chickens were analysed for a correlation between the bioluminescent signal and the number of bacteria. Transposition of the lux operon into the chromosome of the APEC isolate did not affect sensitivity to lytic bacteriophages and there was no effect on virulence in an intratracheal infection model in 1-day-old chicks, although results with a subcutaneous infection model were inconclusive. A correlation between the number of bacteria and the luminescent signal was found in liquid medium, as well as in homogenised heart, liver, spleen and lung of 4-week-old experimentally infected chickens. This study showed that lux could be used for identification of the infecting strain after experimental infection with APEC in poultry. {\textcopyright} 2016 Elsevier Ltd",

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AU - Noppen, Sam

AU - Liekens, Sandra

AU - Lavigne, Rob

AU - Butaye, Patrick

AU - Goddeeris, Bruno M.

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Bioluminescent avian pathogenic Escherichia coli for monitoring colibacillosis in experimentally infected chickens

Abstract

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Research Keywords

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