Biohydrogen production from cellobiose in phenol and cresol-containing medium using Clostridium sp. R1

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Original languageEnglish
Pages (from-to)10239-10244
Journal / PublicationInternational Journal of Hydrogen Energy
Issue number19
Publication statusPublished - Oct 2010
Externally publishedYes


Cellobiose fermentation in batch test using an isolated strain, Clostridium sp. R1, was investigated. The Clostridium sp. R1 achieved a maximum hydrogen yield of 3.5 mol H2 mol-1 cellobiose at pH 6 and 30 °C, higher than most yields reported in literature. This strain can generate hydrogen from a number of carbohydrates, including galactose, glucose, mannose, maltose, sucrose, and starch. This strain can also convert cellobiose to hydrogen in the presence of toxic phenol or cresol. The inhibition effects of phenolic compounds on strain R1 activity followed phenol > p-cresol > o-cresol > m-cresol. Co-culturing with another strain, Clostridium butyricum, can co-degrade some of the phenol as substrates. The new isolated strain can yield hydrogen from phenol-containing wastewaters. © 2010 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.

Research Area(s)

  • Cellobiose, Clostridium, Cresol, Hydrogen, Phenol

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