Biofilm Formation Assay in Pseudomonas syringae

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

15 Scopus Citations
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Detail(s)

Original languageEnglish
Article numbere3237
Journal / PublicationBio-protocol
Volume9
Issue number10
Online published20 May 2019
Publication statusPublished - 2019

Abstract

Pseudomonas syringae is a model plant pathogen that infects more than 50 plant species worldwide, thus leading to significant yield loss. Pseudomonas biofilm always adheres to the surfaces of medical devices or host cells, thereby contributing to infection. Biofilm formation can be visualized on numerous matrixes, including coverslips, silicone tubes, polypropylene and polystyrene. Confocal laser scanning microscopy can be used to visualize and analyze biofilm structure. In this study, we modified and applied the current method of P. aeruginosa biofilm measurement to P. syringae, and developed a convenient protocol to visualize P. syringae biofilm formation using a borosilicate glass tube as the matrix coupled with crystal violet staining.

Research Area(s)

  • Biofilm, Pseudomonas syringae, Plant pathogen, Borosilicate glass, Crystal violet, Visualization, ALGINATE BIOSYNTHESIS, AERUGINOSA, EXPRESSION

Citation Format(s)

Biofilm Formation Assay in Pseudomonas syringae. / Shao, Xiaolong; Xie, Yingpeng; Zhang, Yingchao et al.
In: Bio-protocol, Vol. 9, No. 10, e3237, 2019.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review