Biofilm Formation Assay in Pseudomonas syringae
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
Original language | English |
---|---|
Article number | e3237 |
Journal / Publication | Bio-protocol |
Volume | 9 |
Issue number | 10 |
Online published | 20 May 2019 |
Publication status | Published - 2019 |
Link(s)
Abstract
Pseudomonas syringae is a model plant pathogen that infects more than 50 plant species worldwide, thus leading to significant yield loss. Pseudomonas biofilm always adheres to the surfaces of medical devices or host cells, thereby contributing to infection. Biofilm formation can be visualized on numerous matrixes, including coverslips, silicone tubes, polypropylene and polystyrene. Confocal laser scanning microscopy can be used to visualize and analyze biofilm structure. In this study, we modified and applied the current method of P. aeruginosa biofilm measurement to P. syringae, and developed a convenient protocol to visualize P. syringae biofilm formation using a borosilicate glass tube as the matrix coupled with crystal violet staining.
Research Area(s)
- Biofilm, Pseudomonas syringae, Plant pathogen, Borosilicate glass, Crystal violet, Visualization, ALGINATE BIOSYNTHESIS, AERUGINOSA, EXPRESSION
Citation Format(s)
Biofilm Formation Assay in Pseudomonas syringae. / Shao, Xiaolong; Xie, Yingpeng; Zhang, Yingchao et al.
In: Bio-protocol, Vol. 9, No. 10, e3237, 2019.
In: Bio-protocol, Vol. 9, No. 10, e3237, 2019.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review