Biodegradation and enzymatic responses in the marine diatom Skeletonema costatum upon exposure to 2,4-dichlorophenol

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Original languageEnglish
Pages (from-to)191-200
Journal / PublicationAquatic Toxicology
Issue number3-4
Publication statusPublished - 2002


The biodegradation and responses of selected detoxification and antioxidant enzymes in the marine diatom, Skeletonema costatum, upon exposure to sublethal concentrations of 2,4-dichlorophenol (2,4-DCP) were investigated. Results show that 2,4-DCP was readily metabolised, but bioaccumulation and adsorption were negligible. Glutathione S-transferase, ascorbate peroxidase and superoxide dismutase activities were increased markedly after exposure to 2,4-DCP for 96 h, while no appreciable change in peroxidase activity was observed. The addition of exogeneous glutathione to diatom culture enhanced the degradation of 2,4-DCP, and promoted diatom growth. The inhibition of glutathione synthesis enhanced the toxicity of 2,4-DCP. These results suggest that glutathione conjugation was one of the principal mechanisms involved in the degradation of 2,4-DCP in this diatom. Copyright © 2002 Elsevier Science B.V.

Research Area(s)

  • 2,4-Dichlorophenol, Degradation, Detoxification, Diatom, Enzyme