Bioconversion of styrene to poly(hydroxyalkanoate) (PHA) by the new bacterial strain Pseudomonas putida NBUS12

Giin-Yu Amy Tan; Chia-Lung Chen; Liya Ge; Ling Li; Swee Ngin Tan; Jing-Yuan Wang

doi:10.1264/jsme2.ME14138

Bioconversion of styrene to poly(hydroxyalkanoate) (PHA) by the new bacterial strain Pseudomonas putida NBUS12

Giin-Yu Amy Tan, Chia-Lung Chen, Liya Ge, Ling Li, Swee Ngin Tan, Jing-Yuan Wang^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

39 Citations (Scopus)

Abstract

Styrene is a toxic pollutant commonly found in waste effluents from plastic processing industries. We herein identified and characterized microorganisms for bioconversion of the organic eco-pollutant styrene into a valuable biopolymer mediumchain-length poly(hydroxyalkanoate) (mcl-PHA). Twelve newly-isolated styrene-degrading Pseudomonads were obtained and partial phaC genes were detected by PCR in these isolates. These isolates assimilated styrene to produce mcl-PHA, forming PHA contents between 0.05±0.00 and 23.10±3.25% cell dry mass (% CDM). The best-performing isolate was identified as Pseudomonas putida NBUS12. A genetic analysis of 16S rDNA and phaZ genes revealed P. putida NBUS12 as a geneticallydistinct strain from existing phenotypically-similar bacterial strains. This bacterium achieved a final biomass of 1.28±0.10 g L⁻¹ and PHA content of 32.49±2.40% CDM. The extracted polymer was mainly comprised of 3-hydroxyhexanoate (C₆), 3-hydroxyoctanoate (C₈), 3-hydroxydecanoate (C₁₀), 3-hydroxydodecanoate (C₁₂), and 3-hydroxytetradecanoate (C₁₄) monomers at a ratio of 2:42:1257:17:1. These results collectively suggested that P. putida NBUS12 is a promising candidate for the biotechnological conversion of styrene into mcl-PHA.

Original language	English
Pages (from-to)	76-85
Journal	Microbes and Environments
Volume	30
Issue number	1
DOIs	https://doi.org/10.1264/jsme2.ME14138
Publication status	Published - 2015
Externally published	Yes

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Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Research Keywords

Biopolyester
Eco-pollutants
Medium-chain-length poly(hydroxyalkanoate) (mcl-PHA)
Pseudomonas putida NBUS12
Styrene

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title = "Bioconversion of styrene to poly(hydroxyalkanoate) (PHA) by the new bacterial strain Pseudomonas putida NBUS12",

abstract = "Styrene is a toxic pollutant commonly found in waste effluents from plastic processing industries. We herein identified and characterized microorganisms for bioconversion of the organic eco-pollutant styrene into a valuable biopolymer mediumchain-length poly(hydroxyalkanoate) (mcl-PHA). Twelve newly-isolated styrene-degrading Pseudomonads were obtained and partial phaC genes were detected by PCR in these isolates. These isolates assimilated styrene to produce mcl-PHA, forming PHA contents between 0.05±0.00 and 23.10±3.25% cell dry mass (% CDM). The best-performing isolate was identified as Pseudomonas putida NBUS12. A genetic analysis of 16S rDNA and phaZ genes revealed P. putida NBUS12 as a geneticallydistinct strain from existing phenotypically-similar bacterial strains. This bacterium achieved a final biomass of 1.28±0.10 g L−1 and PHA content of 32.49±2.40% CDM. The extracted polymer was mainly comprised of 3-hydroxyhexanoate (C6), 3-hydroxyoctanoate (C8), 3-hydroxydecanoate (C10), 3-hydroxydodecanoate (C12), and 3-hydroxytetradecanoate (C14) monomers at a ratio of 2:42:1257:17:1. These results collectively suggested that P. putida NBUS12 is a promising candidate for the biotechnological conversion of styrene into mcl-PHA.",

keywords = "Biopolyester, Eco-pollutants, Medium-chain-length poly(hydroxyalkanoate) (mcl-PHA), Pseudomonas putida NBUS12, Styrene",

author = "Tan, {Giin-Yu Amy} and Chia-Lung Chen and Liya Ge and Ling Li and Tan, {Swee Ngin} and Jing-Yuan Wang",

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doi = "10.1264/jsme2.ME14138",

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T1 - Bioconversion of styrene to poly(hydroxyalkanoate) (PHA) by the new bacterial strain Pseudomonas putida NBUS12

AU - Tan, Giin-Yu Amy

AU - Chen, Chia-Lung

AU - Ge, Liya

AU - Li, Ling

AU - Tan, Swee Ngin

AU - Wang, Jing-Yuan

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2015

Y1 - 2015

N2 - Styrene is a toxic pollutant commonly found in waste effluents from plastic processing industries. We herein identified and characterized microorganisms for bioconversion of the organic eco-pollutant styrene into a valuable biopolymer mediumchain-length poly(hydroxyalkanoate) (mcl-PHA). Twelve newly-isolated styrene-degrading Pseudomonads were obtained and partial phaC genes were detected by PCR in these isolates. These isolates assimilated styrene to produce mcl-PHA, forming PHA contents between 0.05±0.00 and 23.10±3.25% cell dry mass (% CDM). The best-performing isolate was identified as Pseudomonas putida NBUS12. A genetic analysis of 16S rDNA and phaZ genes revealed P. putida NBUS12 as a geneticallydistinct strain from existing phenotypically-similar bacterial strains. This bacterium achieved a final biomass of 1.28±0.10 g L−1 and PHA content of 32.49±2.40% CDM. The extracted polymer was mainly comprised of 3-hydroxyhexanoate (C6), 3-hydroxyoctanoate (C8), 3-hydroxydecanoate (C10), 3-hydroxydodecanoate (C12), and 3-hydroxytetradecanoate (C14) monomers at a ratio of 2:42:1257:17:1. These results collectively suggested that P. putida NBUS12 is a promising candidate for the biotechnological conversion of styrene into mcl-PHA.

AB - Styrene is a toxic pollutant commonly found in waste effluents from plastic processing industries. We herein identified and characterized microorganisms for bioconversion of the organic eco-pollutant styrene into a valuable biopolymer mediumchain-length poly(hydroxyalkanoate) (mcl-PHA). Twelve newly-isolated styrene-degrading Pseudomonads were obtained and partial phaC genes were detected by PCR in these isolates. These isolates assimilated styrene to produce mcl-PHA, forming PHA contents between 0.05±0.00 and 23.10±3.25% cell dry mass (% CDM). The best-performing isolate was identified as Pseudomonas putida NBUS12. A genetic analysis of 16S rDNA and phaZ genes revealed P. putida NBUS12 as a geneticallydistinct strain from existing phenotypically-similar bacterial strains. This bacterium achieved a final biomass of 1.28±0.10 g L−1 and PHA content of 32.49±2.40% CDM. The extracted polymer was mainly comprised of 3-hydroxyhexanoate (C6), 3-hydroxyoctanoate (C8), 3-hydroxydecanoate (C10), 3-hydroxydodecanoate (C12), and 3-hydroxytetradecanoate (C14) monomers at a ratio of 2:42:1257:17:1. These results collectively suggested that P. putida NBUS12 is a promising candidate for the biotechnological conversion of styrene into mcl-PHA.

KW - Biopolyester

KW - Eco-pollutants

KW - Medium-chain-length poly(hydroxyalkanoate) (mcl-PHA)

KW - Pseudomonas putida NBUS12

KW - Styrene

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U2 - 10.1264/jsme2.ME14138

DO - 10.1264/jsme2.ME14138

M3 - RGC 21 - Publication in refereed journal

C2 - 25740622

SN - 1342-6311

VL - 30

SP - 76

EP - 85

JO - Microbes and Environments

JF - Microbes and Environments

IS - 1

ER -

Bioconversion of styrene to poly(hydroxyalkanoate) (PHA) by the new bacterial strain Pseudomonas putida NBUS12

Abstract

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Research Keywords

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