Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products

Lei He; Mengyu Ren; Xueqing Chen; Xiaoyun Wang; Shan Li; Jinsi Lin; Chi Liang; Pei Liang; Yue Hu; Huali Lei; Meng Bian; Yan Huang; Zhongdao Wu; Xuerong Li; Xinbing Yu

doi:10.1007/s00436-014-3935-4

Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products

Lei He
, Mengyu Ren
, Xueqing Chen
, Xiaoyun Wang
, Shan Li
, Jinsi Lin
, Chi Liang
, Pei Liang
, Yue Hu
, Huali Lei
, Meng Bian
, Yan Huang
, Zhongdao Wu
, Xuerong Li
, Xinbing Yu

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

24 Citations (Scopus)

Abstract

Clonorchis sinensis has been classified as group I biological carcinogen for cholangiocarcinoma by the World Health Organization. Biological studies on excretory/secretory products (ESPs) enabled us to understand the pathogenesis mechanism of C. sinensis and develop new strategies for the prevention of clonorchiasis. In this study, sequence analysis showed that annexin B30 from C. sinensis (CsANXB30) is composed of four annexin repeats which were characterized by type II and III Ca²⁺-binding sites or KGD motif with the capability of Ca²⁺-binding. In addition, immunoblot assay revealed that recombinant CsANXB30 (rCsANXB30) could be recognized by the sera from rats infected with C. sinensis and the sera from rats immunized by CsESPs. Real-time PCR showed that its transcriptional level was the highest at the stage of metacercaria. Immunofluorescence assay was employed to confirm that CsANXB30 was distributed in the tegument, intestine, and egg of adult worms, as well as the tegument and vitellarium of metacercaria. rCsANXB30 was able to bind phospholipid in a Ca²⁺-dependent manner and human plasminogen in a dose-dependent manner. Moreover, cytokine and antibody measurements indicated that rats subcutaneously immunized with rCsANXB30 developed a strong IL-10 production in spleen cells and a high level of IgG1 isotype, indicating that rCsANXB30 could trigger specific humoral and cellular immune response in rats. The present results implied that CsANXB30 might be involved in a host-parasite interaction and affected the immune response of the host during C. sinensis infection. © 2014 Springer-Verlag.

Original language	English
Pages (from-to)	2743-2755
Journal	Parasitology Research
Volume	113
Issue number	7
DOIs	https://doi.org/10.1007/s00436-014-3935-4
Publication status	Published - Jul 2014
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

This work was supported by the National Key Basic Research and Development Project of China (973 project; No. 2010CB530000), National Natural Science Foundation of China (No. 81101270 and No. 81171602), the National S & T Major Program (2012ZX10004-220), Fundamental Research Funds for the Central Universities of China (No. 3164015) and Guangdong Natural Science Foundation of China (No. S2012010008504).

Research Keywords

Annexin B30
Clonorchis sinensis
Excretory/secretory products
Immunological characterization
Phospholipid and plasminogen-binding

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title = "Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products",

abstract = "Clonorchis sinensis has been classified as group I biological carcinogen for cholangiocarcinoma by the World Health Organization. Biological studies on excretory/secretory products (ESPs) enabled us to understand the pathogenesis mechanism of C. sinensis and develop new strategies for the prevention of clonorchiasis. In this study, sequence analysis showed that annexin B30 from C. sinensis (CsANXB30) is composed of four annexin repeats which were characterized by type II and III Ca2+-binding sites or KGD motif with the capability of Ca2+-binding. In addition, immunoblot assay revealed that recombinant CsANXB30 (rCsANXB30) could be recognized by the sera from rats infected with C. sinensis and the sera from rats immunized by CsESPs. Real-time PCR showed that its transcriptional level was the highest at the stage of metacercaria. Immunofluorescence assay was employed to confirm that CsANXB30 was distributed in the tegument, intestine, and egg of adult worms, as well as the tegument and vitellarium of metacercaria. rCsANXB30 was able to bind phospholipid in a Ca2+-dependent manner and human plasminogen in a dose-dependent manner. Moreover, cytokine and antibody measurements indicated that rats subcutaneously immunized with rCsANXB30 developed a strong IL-10 production in spleen cells and a high level of IgG1 isotype, indicating that rCsANXB30 could trigger specific humoral and cellular immune response in rats. The present results implied that CsANXB30 might be involved in a host-parasite interaction and affected the immune response of the host during C. sinensis infection. {\textcopyright} 2014 Springer-Verlag.",

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author = "Lei He and Mengyu Ren and Xueqing Chen and Xiaoyun Wang and Shan Li and Jinsi Lin and Chi Liang and Pei Liang and Yue Hu and Huali Lei and Meng Bian and Yan Huang and Zhongdao Wu and Xuerong Li and Xinbing Yu",

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T1 - Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products

AU - He, Lei

AU - Ren, Mengyu

AU - Chen, Xueqing

AU - Wang, Xiaoyun

AU - Li, Shan

AU - Lin, Jinsi

AU - Liang, Chi

AU - Liang, Pei

AU - Hu, Yue

AU - Lei, Huali

AU - Bian, Meng

AU - Huang, Yan

AU - Wu, Zhongdao

AU - Li, Xuerong

AU - Yu, Xinbing

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2014/7

Y1 - 2014/7

N2 - Clonorchis sinensis has been classified as group I biological carcinogen for cholangiocarcinoma by the World Health Organization. Biological studies on excretory/secretory products (ESPs) enabled us to understand the pathogenesis mechanism of C. sinensis and develop new strategies for the prevention of clonorchiasis. In this study, sequence analysis showed that annexin B30 from C. sinensis (CsANXB30) is composed of four annexin repeats which were characterized by type II and III Ca2+-binding sites or KGD motif with the capability of Ca2+-binding. In addition, immunoblot assay revealed that recombinant CsANXB30 (rCsANXB30) could be recognized by the sera from rats infected with C. sinensis and the sera from rats immunized by CsESPs. Real-time PCR showed that its transcriptional level was the highest at the stage of metacercaria. Immunofluorescence assay was employed to confirm that CsANXB30 was distributed in the tegument, intestine, and egg of adult worms, as well as the tegument and vitellarium of metacercaria. rCsANXB30 was able to bind phospholipid in a Ca2+-dependent manner and human plasminogen in a dose-dependent manner. Moreover, cytokine and antibody measurements indicated that rats subcutaneously immunized with rCsANXB30 developed a strong IL-10 production in spleen cells and a high level of IgG1 isotype, indicating that rCsANXB30 could trigger specific humoral and cellular immune response in rats. The present results implied that CsANXB30 might be involved in a host-parasite interaction and affected the immune response of the host during C. sinensis infection. © 2014 Springer-Verlag.

AB - Clonorchis sinensis has been classified as group I biological carcinogen for cholangiocarcinoma by the World Health Organization. Biological studies on excretory/secretory products (ESPs) enabled us to understand the pathogenesis mechanism of C. sinensis and develop new strategies for the prevention of clonorchiasis. In this study, sequence analysis showed that annexin B30 from C. sinensis (CsANXB30) is composed of four annexin repeats which were characterized by type II and III Ca2+-binding sites or KGD motif with the capability of Ca2+-binding. In addition, immunoblot assay revealed that recombinant CsANXB30 (rCsANXB30) could be recognized by the sera from rats infected with C. sinensis and the sera from rats immunized by CsESPs. Real-time PCR showed that its transcriptional level was the highest at the stage of metacercaria. Immunofluorescence assay was employed to confirm that CsANXB30 was distributed in the tegument, intestine, and egg of adult worms, as well as the tegument and vitellarium of metacercaria. rCsANXB30 was able to bind phospholipid in a Ca2+-dependent manner and human plasminogen in a dose-dependent manner. Moreover, cytokine and antibody measurements indicated that rats subcutaneously immunized with rCsANXB30 developed a strong IL-10 production in spleen cells and a high level of IgG1 isotype, indicating that rCsANXB30 could trigger specific humoral and cellular immune response in rats. The present results implied that CsANXB30 might be involved in a host-parasite interaction and affected the immune response of the host during C. sinensis infection. © 2014 Springer-Verlag.

KW - Annexin B30

KW - Clonorchis sinensis

KW - Excretory/secretory products

KW - Immunological characterization

KW - Phospholipid and plasminogen-binding

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U2 - 10.1007/s00436-014-3935-4

DO - 10.1007/s00436-014-3935-4

M3 - RGC 21 - Publication in refereed journal

C2 - 24861011

SN - 0932-0113

VL - 113

SP - 2743

EP - 2755

JO - Parasitology Research

JF - Parasitology Research

IS - 7

ER -

Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products

Abstract

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Research Keywords

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