Bacterial inactivation and in situ monitoring of biofilm development on graphene oxide membrane using optical coherence tomography

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

6 Scopus Citations
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Detail(s)

Original languageEnglish
Pages (from-to)22-34
Number of pages13
Journal / PublicationJournal of Membrane Science
Volume564
Online published30 Jun 2018
Publication statusPublished - 15 Oct 2018

Abstract

In an attempt to advance GO-based environmental applications, herein we probed the anti-biofouling properties and mechanisms of graphene oxide (GO) surface coating. A flexible and mechanically stable GO membrane was fabricated using vacuum filtration technique and its ability to inactivate bacterial growth and subsequent biofilm formation was investigated. Our preliminary results authenticate that the GO membrane, owing to its unique physicochemical surface properties, exhibits superior antibacterial activity against planktonic cell proliferation. An optical coherence tomography (OCT)-based nondestructive in situ monitoring of bacterial biofilm evolution and behavior revealed that the GO surface initially inhibited biofilm growth for 24 h under continuous flow conditions but was incapable of completely averting biofilm development under long-term operation (48 h). We further confirmed that the observed biofilm on the GO membrane was highly unstable and reversibly attached and could be conveniently removed from the surface under a gentle rinsing. Finally, we confirmed that upon direct bacterium-GO contact, GO simultaneously induces cellular membrane disruption and oxidative stress followed by degradation/release of intracellular organelles, thereby causing bacterial inactivation or cell death. We believe our findings will offer new understandings into the anti-biofouling properties of GO-coated membranes and highlight their potential for practical application in membrane-based water and wastewater treatment technologies.

Research Area(s)

  • Biofouling, Graphene oxide membrane, In situ monitoring, Optical coherence tomography, Oxidative stress