Automatic bio-MEMS platforms for fast disease diagnosis

Fu-Chun Huang; Chia-Sheng Liao; Chih-Hao Wang; Gwo-Bin Lee

doi:10.1109/robio.2005.246392

Automatic bio-MEMS platforms for fast disease diagnosis

Fu-Chun Huang
, Chia-Sheng Liao
, Chih-Hao Wang
, Gwo-Bin Lee^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

3 Citations (Scopus)

Abstract

The paper reports two platforms for fast diagnosis of infectious diseases using enabling Bio-MEMS (Bio-Micro-Electro-Mechanical-Systems) technology. The first platform uses molecular biology techniques for DNA-based diagnosis. An integrated microfluidic chip capable of performing DNA/RNA amplification, electrokinetic sample injection and separation, and on-line optical detection of nucleic acid products is developed. To demonstrate the functionality of this platform, RNA-based detection for Dengue 2 virus has been preformed in an automatic format with less sample and reagent volumes in a shorter period thanks to lower thermal inertia of the micro systems. The second platform uses ELISA (Enzyme-linked immunosorbent assay) techniques for disease detection by employing an enzyme label for detection of antibody-antigen complexes. An automatic disease diagnosis system could be realized by integrating micropumps and microvalves. HCV (hepatitis C virus) and syphilis serum samples are successfully tested on the developed chips using ELISA procedure. These two Bio-MEMS platforms could be crucial for molecular biology, genetic analysis, infectious disease detection, and other biomedical applications. © 2005 IEEE.

Original language	English
Title of host publication	2005 IEEE International Conference on Robotics and Biomimetics, ROBIO
Pages	10-15
Volume	2005
DOIs	https://doi.org/10.1109/robio.2005.246392
Publication status	Published - 2005
Externally published	Yes
Event	2005 IEEE International Conference on Robotics and Biomimetics (ROBIO 2005) - Hong Kong, China Duration: 5 Jul 2005 → 9 Jul 2005

Publication series

Name	2005 IEEE International Conference on Robotics and Biomimetics, ROBIO
Volume	2005

Conference

Conference	2005 IEEE International Conference on Robotics and Biomimetics (ROBIO 2005)
Place	China
City	Hong Kong
Period	5/07/05 → 9/07/05

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

DNA
Electrophoresis
ELISA
Microfluidics
Micropump
PCR
RNA

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10.1109/robio.2005.246392

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Cite this

@inproceedings{03c51abd16b240b9a760e94768ba72e7,

title = "Automatic bio-MEMS platforms for fast disease diagnosis",

abstract = "The paper reports two platforms for fast diagnosis of infectious diseases using enabling Bio-MEMS (Bio-Micro-Electro-Mechanical-Systems) technology. The first platform uses molecular biology techniques for DNA-based diagnosis. An integrated microfluidic chip capable of performing DNA/RNA amplification, electrokinetic sample injection and separation, and on-line optical detection of nucleic acid products is developed. To demonstrate the functionality of this platform, RNA-based detection for Dengue 2 virus has been preformed in an automatic format with less sample and reagent volumes in a shorter period thanks to lower thermal inertia of the micro systems. The second platform uses ELISA (Enzyme-linked immunosorbent assay) techniques for disease detection by employing an enzyme label for detection of antibody-antigen complexes. An automatic disease diagnosis system could be realized by integrating micropumps and microvalves. HCV (hepatitis C virus) and syphilis serum samples are successfully tested on the developed chips using ELISA procedure. These two Bio-MEMS platforms could be crucial for molecular biology, genetic analysis, infectious disease detection, and other biomedical applications. {\textcopyright} 2005 IEEE.",

keywords = "DNA, Electrophoresis, ELISA, Microfluidics, Micropump, PCR, RNA",

author = "Fu-Chun Huang and Chia-Sheng Liao and Chih-Hao Wang and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 2005 IEEE International Conference on Robotics and Biomimetics (ROBIO 2005) ; Conference date: 05-07-2005 Through 09-07-2005",

year = "2005",

doi = "10.1109/robio.2005.246392",

language = "English",

isbn = "0780393155",

volume = "2005",

series = "2005 IEEE International Conference on Robotics and Biomimetics, ROBIO",

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booktitle = "2005 IEEE International Conference on Robotics and Biomimetics, ROBIO",

}

Huang, F-C, Liao, C-S, Wang, C-H & Lee, G-B 2005, Automatic bio-MEMS platforms for fast disease diagnosis. in 2005 IEEE International Conference on Robotics and Biomimetics, ROBIO. vol. 2005, 1708587, 2005 IEEE International Conference on Robotics and Biomimetics, ROBIO, vol. 2005, pp. 10-15, 2005 IEEE International Conference on Robotics and Biomimetics (ROBIO 2005), Hong Kong, China, 5/07/05. https://doi.org/10.1109/robio.2005.246392

Automatic bio-MEMS platforms for fast disease diagnosis. / Huang, Fu-Chun; Liao, Chia-Sheng; Wang, Chih-Hao et al.
2005 IEEE International Conference on Robotics and Biomimetics, ROBIO. Vol. 2005 2005. p. 10-15 1708587 (2005 IEEE International Conference on Robotics and Biomimetics, ROBIO; Vol. 2005).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Automatic bio-MEMS platforms for fast disease diagnosis

AU - Huang, Fu-Chun

AU - Liao, Chia-Sheng

AU - Wang, Chih-Hao

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2005

Y1 - 2005

N2 - The paper reports two platforms for fast diagnosis of infectious diseases using enabling Bio-MEMS (Bio-Micro-Electro-Mechanical-Systems) technology. The first platform uses molecular biology techniques for DNA-based diagnosis. An integrated microfluidic chip capable of performing DNA/RNA amplification, electrokinetic sample injection and separation, and on-line optical detection of nucleic acid products is developed. To demonstrate the functionality of this platform, RNA-based detection for Dengue 2 virus has been preformed in an automatic format with less sample and reagent volumes in a shorter period thanks to lower thermal inertia of the micro systems. The second platform uses ELISA (Enzyme-linked immunosorbent assay) techniques for disease detection by employing an enzyme label for detection of antibody-antigen complexes. An automatic disease diagnosis system could be realized by integrating micropumps and microvalves. HCV (hepatitis C virus) and syphilis serum samples are successfully tested on the developed chips using ELISA procedure. These two Bio-MEMS platforms could be crucial for molecular biology, genetic analysis, infectious disease detection, and other biomedical applications. © 2005 IEEE.

AB - The paper reports two platforms for fast diagnosis of infectious diseases using enabling Bio-MEMS (Bio-Micro-Electro-Mechanical-Systems) technology. The first platform uses molecular biology techniques for DNA-based diagnosis. An integrated microfluidic chip capable of performing DNA/RNA amplification, electrokinetic sample injection and separation, and on-line optical detection of nucleic acid products is developed. To demonstrate the functionality of this platform, RNA-based detection for Dengue 2 virus has been preformed in an automatic format with less sample and reagent volumes in a shorter period thanks to lower thermal inertia of the micro systems. The second platform uses ELISA (Enzyme-linked immunosorbent assay) techniques for disease detection by employing an enzyme label for detection of antibody-antigen complexes. An automatic disease diagnosis system could be realized by integrating micropumps and microvalves. HCV (hepatitis C virus) and syphilis serum samples are successfully tested on the developed chips using ELISA procedure. These two Bio-MEMS platforms could be crucial for molecular biology, genetic analysis, infectious disease detection, and other biomedical applications. © 2005 IEEE.

KW - DNA

KW - Electrophoresis

KW - ELISA

KW - Microfluidics

KW - Micropump

KW - PCR

KW - RNA

UR - https://www.scopus.com/pages/publications/33947705670

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-33947705670&origin=recordpage

U2 - 10.1109/robio.2005.246392

DO - 10.1109/robio.2005.246392

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 0780393155

SN - 9780780393158

VL - 2005

T3 - 2005 IEEE International Conference on Robotics and Biomimetics, ROBIO

SP - 10

EP - 15

BT - 2005 IEEE International Conference on Robotics and Biomimetics, ROBIO

T2 - 2005 IEEE International Conference on Robotics and Biomimetics (ROBIO 2005)

Y2 - 5 July 2005 through 9 July 2005

ER -

Automatic bio-MEMS platforms for fast disease diagnosis

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Research Keywords

Access Output

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Permanent Link

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