Automated Parallel Electrical Characterization of Cells Using Optically-Induced Dielectrophoresis

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

12 Scopus Citations
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Author(s)

  • Na Liu
  • Yanbin Lin
  • Yan Peng
  • Liming Xin
  • Tao Yue
  • Yuanyuan Liu
  • Changhai Ru
  • Shaorong Xie
  • Liang Dong
  • Huayan Pu
  • Haige Chen
  • Yu Sun

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article number8984249
Pages (from-to)1084-1092
Journal / PublicationIEEE Transactions on Automation Science and Engineering
Volume17
Issue number2
Online published5 Feb 2020
Publication statusPublished - Apr 2020

Abstract

This article reports an automated optically-induced dielectrophoresis (ODEP) system for characterizing the specific membrane capacitance (SMC) of individual cells. The simulation of cell motion is conducted to analyze the electrokinetic forces acting on the cell. A self-developed visual tracking algorithm for multicells is used to realize an automated process for determining the frequency-sweeping range, crossover frequencies, and cell radii. The SMC values of malignant bladder cancer cells (T24 and RT4) and normal urothelial cells (SV-HUC-1) were quantified using the automated system, demonstrating that the system has a measurement speed of 1 cell/s, an accuracy of 1 kHz for the crossover frequency determination, and an accuracy of 0.2 μm for the cell radius measurement.

Research Area(s)

  • Automated measurement, biological cell, optically-induced dielectrophoresis (ODEP), specific membrane capacitance (SMC)

Citation Format(s)

Automated Parallel Electrical Characterization of Cells Using Optically-Induced Dielectrophoresis. / Liu, Na; Lin, Yanbin; Peng, Yan; Xin, Liming; Yue, Tao; Liu, Yuanyuan; Ru, Changhai; Xie, Shaorong; Dong, Liang; Pu, Huayan; Chen, Haige; Li, Wen J.; Sun, Yu.

In: IEEE Transactions on Automation Science and Engineering, Vol. 17, No. 2, 8984249, 04.2020, p. 1084-1092.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review