Abstract
The determination of albendazole (ABZ) and its metabolites, albendazole sulfoxide, albendazole sulfone, and albendazole 2-aminosulfone in crab tissue is a complex analysis involving the extraction of trace levels of potentially unstable analytes from a solid matrix. Here, we report on an online solid-phase extraction (SPE) for automated trapping of ABZ and its metabolites in Chinese mitten crab, Eriocheir sinensis H. Milne Edwards (E. sinensis), followed by stable isotope dilution UPLC-MS/MS analysis. The performance of online SPE was fully validated. Good linearity was obtained with correlation coefficients >0. 99. In the analytical range, the intra-day and inter-day precision and accuracies (RSD %) were 1. 44-4. 62 and 3. 28-6. 93 %, respectively. The mean recoveries were in the range of 78. 2-109. 3 %. All these demonstrated that the online SPE is an excellent technique for trapping and purification of ABZ and its metabolites in crab tissue samples. Finally, this online SPE-UPLC-MS/MS strategy was successfully applied to analyze the crude extracts from several crab tissue samples. © Springer-Verlag Berlin Heidelberg 2013.
| Original language | English |
|---|---|
| Pages (from-to) | 919-929 |
| Journal | European Food Research and Technology |
| Volume | 236 |
| Issue number | 5 |
| Online published | 20 Mar 2013 |
| DOIs | |
| Publication status | Published - May 2013 |
Research Keywords
- Albendazole
- Eriocheir sinensis H. Milne Edwards
- Online solid-phase extraction
- Stable isotope dilution
- Ultra-performance liquid chromatography/mass spectrometry
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