Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow

Terence T. W. Wong; Andy K. S. Lau; Kenneth K. Y. Ho; Matthew Y. H. Tang; Joseph D. F. Robles; Xiaoming Wei; Antony C. S. Chan; Anson H. L. Tang; Edmund Y. Lam; Kenneth K. Y. Wong; Godfrey C. F. Chan; Ho Cheung Shum; Kevin K. Tsia

doi:10.1038/srep03656

Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow

Terence T. W. Wong, Andy K. S. Lau, Kenneth K. Y. Ho, Matthew Y. H. Tang, Joseph D. F. Robles, Xiaoming Wei, Antony C. S. Chan, Anson H. L. Tang, Edmund Y. Lam, Kenneth K. Y. Wong, Godfrey C. F. Chan, Ho Cheung Shum, Kevin K. Tsia

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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Abstract

Accelerating imaging speed in optical microscopy is often realized at the expense of image contrast, image resolution, and detection sensitivity - a common predicament for advancing high-speed and high-throughput cellular imaging. We here demonstrate a new imaging approach, called asymmetric-detection time-stretch optical microscopy (ATOM), which can deliver ultrafast label-free high-contrast flow imaging with well delineated cellular morphological resolution and in-line optical image amplification to overcome the compromised imaging sensitivity at high speed. We show that ATOM can separately reveal the enhanced phase-gradient and absorption contrast in microfluidic live-cell imaging at a flow speed as high as ∼10 m/s, corresponding to an imaging throughput of ∼100,000 cells/sec. ATOM could thus be the enabling platform to meet the pressing need for intercalating optical microscopy in cellular assay, e.g. imaging flow cytometry - permitting high-throughput access to the morphological information of the individual cells simultaneously with a multitude of parameters obtained in the standard assay.

Original language	English
Article number	3656
Journal	Scientific Reports
Volume	4
DOIs	https://doi.org/10.1038/srep03656
Publication status	Published - 13 Jan 2014
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

We thank Hilary K.Y. Mak for preparing the MIHA, BEL-7402 and HeLa cell lines for us. This work was partially supported by grant from the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. HKU 7172/12E, HKU 717510E, HKU 717911E, HKU 720112E) and University Development Fund of HKU. H.C. Shum was supported in part by the Young Scholar's Program (NSFC51206138/E0605) from the National Natural Science Foundation of China and the Basic Research Program-General Program (JC201105190878A) from the Science and Technology Innovation Commission of Shenzhen Municipality.

Publisher's Copyright Statement

This full text is made available under CC-BY-NC-ND 3.0. https://creativecommons.org/licenses/by-nc-nd/3.0/

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Wong, T. T. W., Lau, A. K. S., Ho, K. K. Y., Tang, M. Y. H., Robles, J. D. F., Wei, X., Chan, A. C. S., Tang, A. H. L., Lam, E. Y., Wong, K. K. Y., Chan, G. C. F., Shum, H. C., & Tsia, K. K. (2014). Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow. Scientific Reports, 4, Article 3656. https://doi.org/10.1038/srep03656

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abstract = "Accelerating imaging speed in optical microscopy is often realized at the expense of image contrast, image resolution, and detection sensitivity - a common predicament for advancing high-speed and high-throughput cellular imaging. We here demonstrate a new imaging approach, called asymmetric-detection time-stretch optical microscopy (ATOM), which can deliver ultrafast label-free high-contrast flow imaging with well delineated cellular morphological resolution and in-line optical image amplification to overcome the compromised imaging sensitivity at high speed. We show that ATOM can separately reveal the enhanced phase-gradient and absorption contrast in microfluidic live-cell imaging at a flow speed as high as ∼10 m/s, corresponding to an imaging throughput of ∼100,000 cells/sec. ATOM could thus be the enabling platform to meet the pressing need for intercalating optical microscopy in cellular assay, e.g. imaging flow cytometry - permitting high-throughput access to the morphological information of the individual cells simultaneously with a multitude of parameters obtained in the standard assay.",

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Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow

Abstract

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